Junde Zhou1, Linna Wang2, Yunfu Cui3, Lanhua Tang4. 1. Ward 3 of General Surgery, The Second Affiliated Hospital of Harbin Medical University Harbin 150086, Heilongjiang, China. 2. The Sixth Department of Oncology, General Hospital of Heilongjiang General Bureau of Land Reclamation Harbin 150088, Heilongjiang, China. 3. Ward 1 of General Surgery, The Second Affiliated Hospital of Harbin Medical University Harbin 150086, Heilongjiang, China. 4. Department of Oncology, Xiangya Hospital, Central South University Changsha 410008, Hunan, China.
Abstract
OBJECTIVE: To explore the regulation of miR-125a-5p in hepatocellular carcinoma (HCC) and its mechanisms. METHODS: By transfecting a miR-125a-5p sequence and an interfering sequence of miR-125a-5p-s into human HCC cell lines HCC-LM3 and HepG2, miR-125a-5p-related levels were assesed by Western blot. The abilities of cell proliferation and migration were assessed by cell culture and Transwell assay, respectively. RESULTS: HepG2 cells showed increased miR-125a-5p levels compared with HCC-LM3 cells (P < 0.01). However, compared with QZG cells, the level of miR-125a-5p in HepG2 and HCC-LM3 cells was down-regulated. Compared with miR-125a-5p groups, miR-125a-5p-s groups showed increased colony formation rate and mobility (P < 0.01). After being transfected with miR-125a-5p, the transformation factor 2β (TRA2β) and mRNA levels were decreased, whereas 5p-s expression was increased (P < 0.01). Inhibition of TRA2β by small interfering RNA (siRNA) diminished the ability of cells. CONCLUSION: miR-125a-5p inhibits the invasive capacity of HCC cells through targeting the TRA2β pathway. AJTR
OBJECTIVE: To explore the regulation of miR-125a-5p in hepatocellular carcinoma (HCC) and its mechanisms. METHODS: By transfecting a miR-125a-5p sequence and an interfering sequence of miR-125a-5p-s into human HCC cell lines HCC-LM3 and HepG2, miR-125a-5p-related levels were assesed by Western blot. The abilities of cell proliferation and migration were assessed by cell culture and Transwell assay, respectively. RESULTS: HepG2 cells showed increased miR-125a-5p levels compared with HCC-LM3 cells (P < 0.01). However, compared with QZG cells, the level of miR-125a-5p in HepG2 and HCC-LM3 cells was down-regulated. Compared with miR-125a-5p groups, miR-125a-5p-s groups showed increased colony formation rate and mobility (P < 0.01). After being transfected with miR-125a-5p, the transformation factor 2β (TRA2β) and mRNA levels were decreased, whereas 5p-s expression was increased (P < 0.01). Inhibition of TRA2β by small interfering RNA (siRNA) diminished the ability of cells. CONCLUSION: miR-125a-5p inhibits the invasive capacity of HCC cells through targeting the TRA2β pathway. AJTR
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