An analysis of the characteristics of the intestinal flora in patients with Parkinson's disease complicated with constipation.

Parkinson's disease (PD) is a degenerative disease of the central nervous system (CNS) and is common among the middle-aged and elderly populations. Increasing evidence shows that the gut microbiota may trigger PD through the "gut-microbiota-brain" axis. A previous study revealed that constipation, one of the non-motor symptoms of PD, affects gut microbiota and the progression of PD. However, whether constipation is involved in gut microbiota-associated PD is largely unknown. Therefore, we investigated the relationship between gut microbiota, PD, and constipation in this study. We carried out 16S rRNA sequencing in 15 constipated PD patients (C-PD), 14 non-constipated PD (NC-PD) patients, and 15 healthy controls to evaluate the microbial population. Furthermore, co-occurrence networks were used to assess the gut ecology of the three groups. Spearman analyses were used to analyze the correlation between the differential microbiota and the clinical features. The results showed that there were differences in the composition of the gut microbiota among the C-PD group, the NC-PD group, and the healthy controls. No significant differences were observed in the alpha diversity among the three groups, but the beta diversity differed significantly among the groups. Compared with the healthy controls, the abundance of Hungatella and Collinsella was increased and the abundance of Lachnospira and Fusicatenibacter was reduced in the PD patients' feces. Compared with the NC-PD group, the relative abundance of Megamonas and Holdemanella were lower, while Hungatella, Streptococcus and Anaerotruncus were enriched in the C-PD group. The co-occurrence network analysis showed that the C-PD group presented a different microbial community relationship compared with the NC-PD group and the healthy controls. Our study provides strong evidence that the gut microbiota may be related to constipation in PD. In addition, our data suggest an association between the differential microbiota genera and the clinical features of PD. Therefore, modulating gut microbiota may be another way to monitor and optimize PD treatment. AJTR