Sophia Letsiou1, Ioannis Ganopoulos2, Aliki Kapazoglou3, Aliki Xanthopoulou2,4, Eirini Sarrou3, Georgia Tanou5,6, Athanassios Molassiotis2. 1. Institute of Plant Breeding and Genetic Resources, ELGO-DEMETER, 57001, Thessaloniki-Thermi, Greece. sletsiou@gmail.com. 2. Institute of Plant Breeding and Genetic Resources, ELGO-DEMETER, 57001, Thessaloniki-Thermi, Greece. 3. Institute of Olive Tree, Subtropical Crops and Viticulture (IOSV), Department of Vitis, ELGO-DEMETER, Lykovrysi, 14123, Athens, Greece. 4. Laboratory of Pomology, Department of Horticulture, Aristotle University of Thessaloniki, 57001, Thessaloniki-Thermi, Greece. 5. Institute of Soil and Water Resources, ELGO-DEMETER, 57001, Thessaloniki-Thermi, Greece. 6. Joint Laboratory of Horticulture, ELGO-DEMETER, 57001, Thessaloniki-Thermi, Greece.
Abstract
BACKGROUND: Natural products are not only positioned in the heart of traditional medicine but also in modern medicine as many current drugs are coming from natural sources. Apart from the field of medicine and therapeutics, natural products are broadly used in other industrial fields such as nutrition, skincare products and nanotechnology. METHODS AND RESULTS: The aim of this study was to assess the effects of sweet cherry (Prunus avium L.) fruit extract from the Greek native cultivar 'Vasiliadi', on the human 2D and 3D in vitro models in order to investigate its potential impact on skin. We focused on 2D culture of primary normal human epidermal keratinocytes (NHEK) that were treated with sweet cherry fruit extract. In the first place, we targeted fruit extract potential cytotoxicity by determining ATP intracellular levels. Furthermore, we assessed its potential skin irritability by using 3D skin model. To better understand the bioactivity of sweet cherry fruit. extract, we used qPCR to study the expression of various genes that are implicated in the skin functions. Our experiments showed that sweet cherry fruit extract is non-toxic in 2D keratinocytes culture as well as non-irritant in 3D skin model. Our results revealed that the extract mediated important pathways for the optimum epidermis function such as cell proliferation, immune and inflammatory response. CONCLUSION: The sweet cherry fruit extracts possesses significant activity in epidermis function without any potential of cytotoxicity or skin irritability, which makes it a rather promising active agent for skincare.
BACKGROUND: Natural products are not only positioned in the heart of traditional medicine but also in modern medicine as many current drugs are coming from natural sources. Apart from the field of medicine and therapeutics, natural products are broadly used in other industrial fields such as nutrition, skincare products and nanotechnology. METHODS AND RESULTS: The aim of this study was to assess the effects of sweet cherry (Prunus avium L.) fruit extract from the Greek native cultivar 'Vasiliadi', on the human 2D and 3D in vitro models in order to investigate its potential impact on skin. We focused on 2D culture of primary normal human epidermal keratinocytes (NHEK) that were treated with sweet cherry fruit extract. In the first place, we targeted fruit extract potential cytotoxicity by determining ATP intracellular levels. Furthermore, we assessed its potential skin irritability by using 3D skin model. To better understand the bioactivity of sweet cherry fruit. extract, we used qPCR to study the expression of various genes that are implicated in the skin functions. Our experiments showed that sweet cherry fruit extract is non-toxic in 2D keratinocytes culture as well as non-irritant in 3D skin model. Our results revealed that the extract mediated important pathways for the optimum epidermis function such as cell proliferation, immune and inflammatory response. CONCLUSION: The sweet cherry fruit extracts possesses significant activity in epidermis function without any potential of cytotoxicity or skin irritability, which makes it a rather promising active agent for skincare.
Authors: Claudete Bastos; Lillian Barros; Montserrat Dueñas; Ricardo C Calhelha; Maria João R P Queiroz; Celestino Santos-Buelga; Isabel C F R Ferreira Journal: Food Chem Date: 2014-11-03 Impact factor: 7.514
Authors: Beatrice Dyring-Andersen; Marianne Bengtson Løvendorf; Fabian Coscia; Alberto Santos; Line Bruun Pilgaard Møller; Ana R Colaço; Lili Niu; Michael Bzorek; Sophia Doll; Jørgen Lock Andersen; Rachael A Clark; Lone Skov; Marcel B M Teunissen; Matthias Mann Journal: Nat Commun Date: 2020-11-05 Impact factor: 14.919