Gizem Aytogu1, Eda B Toker1, Ozkan Yavas2, Berfin Kadiroglu1, Ozer Ates1, Musa Ozgur Ozyigit2, Kadir Yesilbag3. 1. Department of Virology, Faculty of Veterinary Medicine, Bursa Uludag University, 16059, Bursa, Turkey. 2. Department of Pathology, Faculty of Veterinary Medicine, Bursa Uludag University, 16059, Bursa, Turkey. 3. Department of Virology, Faculty of Veterinary Medicine, Bursa Uludag University, 16059, Bursa, Turkey. kyesilbag@uludag.edu.tr.
Abstract
BACKGROUND: Pigs are the main host species for the pseudorabies virus. It causes fatal encephalitis in many species, including humans. This article aims to report the first clinical case of pseudorabies as well as isolation and molecular characterization of the virus from a hunting dog in Bursa province, Turkey. METHODS AND RESULTS: The dog shows clinical signs including pruritus and neurological signs such as stumbling and inability to stand up compatible with pseudorabies. The virus isolates were obtained from the supernatant of fresh tissue samples from the cerebellum, cornu ammonis, spleen, salivary gland, conjunctival swab, serum, and PBMC samples. The glycoprotein C region is targeted for viral DNA amplification. Pseudorabies virus genome detected both in fresh tissues and supernatants of third passage on Vero cells. The number of PCR positive samples was dramatically increased after cell culture inoculations. Genome sequencing of strain Bursa-10303, which was isolated from a non-endemic area, identified it to belong to clade A. CONCLUSIONS: This study confirms the possible presence of pseudorabies infection in the wildlife reservoirs in Turkey. Future studies may clarify the importance of the infection in Turkey region, where there is no prevalent pig production.
BACKGROUND: Pigs are the main host species for the pseudorabies virus. It causes fatal encephalitis in many species, including humans. This article aims to report the first clinical case of pseudorabies as well as isolation and molecular characterization of the virus from a hunting dog in Bursa province, Turkey. METHODS AND RESULTS: The dog shows clinical signs including pruritus and neurological signs such as stumbling and inability to stand up compatible with pseudorabies. The virus isolates were obtained from the supernatant of fresh tissue samples from the cerebellum, cornu ammonis, spleen, salivary gland, conjunctival swab, serum, and PBMC samples. The glycoprotein C region is targeted for viral DNA amplification. Pseudorabies virus genome detected both in fresh tissues and supernatants of third passage on Vero cells. The number of PCR positive samples was dramatically increased after cell culture inoculations. Genome sequencing of strain Bursa-10303, which was isolated from a non-endemic area, identified it to belong to clade A. CONCLUSIONS: This study confirms the possible presence of pseudorabies infection in the wildlife reservoirs in Turkey. Future studies may clarify the importance of the infection in Turkey region, where there is no prevalent pig production.
Authors: D Cano-Terriza; R Martínez; A Moreno; J E Pérez-Marín; S Jiménez-Ruiz; J Paniagua; C Borge; I García-Bocanegra Journal: Ecohealth Date: 2019-07-12 Impact factor: 3.184
Authors: Maria Soledad Serena; Germán Ernesto Metz; Maria Ines Lozada; Carolina Gabriela Aspitia; Edgardo Héctor Nicolino; Claudio Luis Pidone; Melisa Fossaroli; Agustin Balsalobre; Maria Alejandra Quiroga; Maria Gabriela Echeverria Journal: Open Vet J Date: 2018-04-11
Authors: Kerri Pedersen; Clinton T Turnage; Wesson D Gaston; Paulo Arruda; Scott A Alls; Thomas Gidlewski Journal: BMC Vet Res Date: 2018-12-06 Impact factor: 2.741