Biocompatible Three-Dimensional Hydrogel Cell Scaffold Fabricated by Sodium Hyaluronate and Chitosan Assisted Two-Photon Polymerization.

The biocompatibility of the three-dimensional (3D) hydrogel cell scaffolds that sodium hyaluronate (SH) and carboxymethyl chitosan (Chitosan) has been investigated. The minimum processing threshold of 2.94 mW and the feature line width of 80 nm have been obtained by the two-photon polymerization (TPP) technology using the as-prepared biocompatible photoresist. Through tuning the contents of the cross-linker in the hydrogel, the mechanical property of 3D hydrogel scaffold can be optimized to ensure the stability and affinity of scaffold. Furthermore, we have fabricated the SH and Chitosan assisted 3D hydrogel cell scaffolds and investigated the biocompatibility. This study provides a facile method to develop 3D biocompatible hydrogel cell scaffold, which would open up new avenues for the potential application in tissue engineering.