Mohamed El-Telbany1, Ahmed El-Sharaki2. 1. Department of Microbiology and Botany, Faculty of Science, Zagazig University, 44519, Zagazig, Egypt. 2. Department of Oral Medicine and Periodontology, Faculty of Dentistry, Zagazig University, 44519, Zagazig, Egypt.
Abstract
AIM: The aim of this study was to isolate multi-drug-resistant p. aeruginosa from dental implant, and control the growth and biofilm of isolated p. aeruginosa by silver nanoparticles. MATERIALS AND METHODS: Thirty specimens from patients with Peri-implantitis were taken for isolation of p. aeruginosa. Bacterial samples were obtained from the infected peri-implant pocket with sterile paper points (size 30-45 mm). Samples were cultured for isolation of Multi-drug resistance P. aeruginosa. Phenotypical identification was done by the VITEK 2 system. DNA was extracted from the isolates and 16S rDNA-based PCR assay was used to confirm the identification. Susceptibility of isolated p. aeruginosa to 16 antibiotics was evaluated using the VITEK 2 system. The growth inhibition of isolated bacteria by AgNPs was tested by disk-diffusion method. The microtiter plate assay was used to estimate the capacity of P. aeruginosa to from biofilms. Antibiofilm activity of AgNPs was determined by microtiter plate assay. RESULTS: Three P. aeruginosa were successfully isolated from 30 clinical specimens. P. aeruginoas isolates were resistance to most of used antibiotics. Silver nanoparticles exerted an inhibitory effect on all isolated bacteria. All tested concentration of AgNPS exhibited a greatest anti-biofilm activity against multi-drug resistance (MDR) p. aeruginosa. CONCLUSION: Current findings highlight the role of AgNPS in growth inhibition of P. aeruginosa and reveal a potential application of AgNPS in eradication of p. aeruginosa biofilms.
AIM: The aim of this study was to isolate multi-drug-resistant p. aeruginosa from dental implant, and control the growth and biofilm of isolated p. aeruginosa by silver nanoparticles. MATERIALS AND METHODS: Thirty specimens from patients with Peri-implantitis were taken for isolation of p. aeruginosa. Bacterial samples were obtained from the infected peri-implant pocket with sterile paper points (size 30-45 mm). Samples were cultured for isolation of Multi-drug resistance P. aeruginosa. Phenotypical identification was done by the VITEK 2 system. DNA was extracted from the isolates and 16S rDNA-based PCR assay was used to confirm the identification. Susceptibility of isolated p. aeruginosa to 16 antibiotics was evaluated using the VITEK 2 system. The growth inhibition of isolated bacteria by AgNPs was tested by disk-diffusion method. The microtiter plate assay was used to estimate the capacity of P. aeruginosa to from biofilms. Antibiofilm activity of AgNPs was determined by microtiter plate assay. RESULTS: Three P. aeruginosa were successfully isolated from 30 clinical specimens. P. aeruginoas isolates were resistance to most of used antibiotics. Silver nanoparticles exerted an inhibitory effect on all isolated bacteria. All tested concentration of AgNPS exhibited a greatest anti-biofilm activity against multi-drug resistance (MDR) p. aeruginosa. CONCLUSION: Current findings highlight the role of AgNPS in growth inhibition of P. aeruginosa and reveal a potential application of AgNPS in eradication of p. aeruginosa biofilms.