A novel method of creating thermoplastic chitosan blends to produce cell scaffolds by FDM additive manufacturing.

Due to its remarkable and promising biological and structural properties, chitosan has been widely studied in several potential applications in the biomedical sector. Attempts are being made to use this polymer and its properties in thermoplastics dedicated to 3D printing in FDM technology. However, chitosan can be processed only from acid solution, which limits its applications. The paper presents a new path for the production of filaments based on unstable chitosan hydrogel obtained by carbon dioxide saturation, as well as synthetic polymers such as polyvinyl alcohol and polycaprolactone. The results confirm that the absence of acid allows formation of thermally stable and printable filaments containing from 5% to 15% of chitosan, capable of reducing S. aureus and E. coli bacteria by 0.41-1.43 in logarithmic scale (56-94%) and 0.28-0.94 in logarithmic scale (36-89%), respectively.