Qianying Guo1,2,3,4,5, Qiang Liu1,2,3,4,5, Nan Wang1,2,3,4,5, Jing Wang1,2,3,4,5, Andi Sun1,2,3,4,5, Jie Qiao1,2,3,4,5, Liying Yan6,7,8,9,10. 1. Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China. 2. National Clinical Research Center for Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China. 3. Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education Beijing Key, Beijing, 100191, China. 4. Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China. 5. Research Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest, Chinese Academy of Medical Sciences, Beijing, China. 6. Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China. yanliyingkind@aliyun.com. 7. National Clinical Research Center for Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China. yanliyingkind@aliyun.com. 8. Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education Beijing Key, Beijing, 100191, China. yanliyingkind@aliyun.com. 9. Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, 100191, China. yanliyingkind@aliyun.com. 10. Research Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest, Chinese Academy of Medical Sciences, Beijing, China. yanliyingkind@aliyun.com.
Abstract
PURPOSE: Nucleoporin 37 (NUP37) has been reported to activate the YAP-TEAD signaling, which is crucial for early embryo development. However, whether NUP37 is involved in oocyte meiosis and embryo development remains largely unknown. The study aimed to clarify the function of Nup37 in oocyte maturation and early embryo development, and to explore the mechanism. METHODS: The expression level and subcellular localization of NUP37 were explored. After knocking down of Nup37 by microinjecting interfering RNA (siRNA), the oocyte maturation rate, aberrant PB1 extrusion rate, and blastocyst formation rate were evaluated. In addition, the effect of the downregulation of Nup37 on YAP-TEAD signaling was confirmed by immunofluorescence staining and real-time quantitative PCR. RESULTS: NUP37 was highly expressed in oocytes and early embryos; it mainly localized to the nuclear periphery at mice GV stage oocytes and early embryos. Nup37 depletion led to aberrant PB1 extrusion at the MII stage oocyte and a decreased blastocyst formation rate. The reduction of NUP37 caused YAP1 mislocalization and decreased the expression of Tead1, Tead2, and Tead4 during mice embryo development, thus affecting the YAP-TEAD activity and embryo developmental competence. CONCLUSIONS: In summary, NUP37 played an important role in mice oocyte maturation and preimplantation embryo development.
PURPOSE: Nucleoporin 37 (NUP37) has been reported to activate the YAP-TEAD signaling, which is crucial for early embryo development. However, whether NUP37 is involved in oocyte meiosis and embryo development remains largely unknown. The study aimed to clarify the function of Nup37 in oocyte maturation and early embryo development, and to explore the mechanism. METHODS: The expression level and subcellular localization of NUP37 were explored. After knocking down of Nup37 by microinjecting interfering RNA (siRNA), the oocyte maturation rate, aberrant PB1 extrusion rate, and blastocyst formation rate were evaluated. In addition, the effect of the downregulation of Nup37 on YAP-TEAD signaling was confirmed by immunofluorescence staining and real-time quantitative PCR. RESULTS: NUP37 was highly expressed in oocytes and early embryos; it mainly localized to the nuclear periphery at mice GV stage oocytes and early embryos. Nup37 depletion led to aberrant PB1 extrusion at the MII stage oocyte and a decreased blastocyst formation rate. The reduction of NUP37 caused YAP1 mislocalization and decreased the expression of Tead1, Tead2, and Tead4 during mice embryo development, thus affecting the YAP-TEAD activity and embryo developmental competence. CONCLUSIONS: In summary, NUP37 played an important role in mice oocyte maturation and preimplantation embryo development.
Authors: Yao Xu; Yingli Shi; Jing Fu; Min Yu; Ruizhi Feng; Qing Sang; Bo Liang; Biaobang Chen; Ronggui Qu; Bin Li; Zheng Yan; Xiaoyan Mao; Yanping Kuang; Li Jin; Lin He; Xiaoxi Sun; Lei Wang Journal: Am J Hum Genet Date: 2016-08-18 Impact factor: 11.025