Literature DB >> 3501861

A rapid method for isolation of double stranded RNA.

J Flegr1.   

Abstract

A rapid and simple method for the isolation and purification of dsRNA is presented. The crucial step of this method is the extraction of proteins and DNA with acid phenol. After the extraction, only RNA is left in the aqueous phase. ssRNA contamination of the RNA preparation can be greatly reduced when ammonium sulfate is present during the extraction.

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Year:  1987        PMID: 3501861     DOI: 10.1080/00327488708062505

Source DB:  PubMed          Journal:  Prep Biochem        ISSN: 0032-7484


  2 in total

1.  Multiple populations of double-stranded RNA in two virus-harbouring strains of Trichomonas vaginalis.

Authors:  J Flegr; J Cerkasov; J Stokrová
Journal:  Folia Microbiol (Praha)       Date:  1988       Impact factor: 2.099

2.  Rapid isolation of mycoviral double-stranded RNA from Botrytis cinerea and Saccharomyces cerevisiae.

Authors:  Antonio Castillo; Luis Cottet; Miguel Castro; Felipe Sepúlveda
Journal:  Virol J       Date:  2011-01-25       Impact factor: 4.099

  2 in total

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