Azo-Based Hypoxia-Responsive Self-Assembly Near-Infrared Fluorescent Nanoprobe for In Vivo Real-Time Bioimaging of Tumors.

Hypoxia is an obvious characteristic of cancer, especially solid tumors. which may give rise to the expansion of invasion and metastasis. Exploring near-infrared (NIR) nanoprobes that could accurately evaluate the degree of hypoxia will contribute to the assessment of the degree of malignant neoplasms, so as to adopt more accurate and individualized treatment options Here, we have developed a self-assembled NIR organic nanoprobe to specifically and authoritatively detect the oxygen concentration in vivo and in vitro to evaluate the level of hypoxia. The organic nanoprobe mainly contains two motifs: a fluorophore moiety NRh-NH2 for NIR fluorescence imaging and hypoxia-sensitive moiety Azonaphthalene derivatives for quenching NIR emissions, detecting oxygen in hypoxic regions and improving the hydrophilicity. The nanoprobes were used for detection of oxygen in a variety of living cells under different conditions and real-time bioimaging of neoplasms in live mice. This design strategy provides ideas for the development of nanoprobes for the diagnosis of tumors and other hypoxia-related diseases.