Pedro Mendoza-de Gives1, Marilem Rodríguez-Labastida2, Agustín Olmedo-Juárez1, María Marcela Gamboa-Angulo3, Manuela Reyes-Estebanez4. 1. Unidad de Investigación en Helmintología, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Boulevar Paseo Cuauhnahuac No. 8534, Col. Progreso, 62550, Jiutepec, Morelos, Mexico. 2. Unidad de Biotecnología, Centro de Investigación Científica de Yucatán A.C., Calle 43 No. 130 x 32 y 34, Chuburná de Hidalgo, 97205, Mérida, Yucatán, Mexico. 3. Unidad de Biotecnología, Centro de Investigación Científica de Yucatán A.C., Calle 43 No. 130 x 32 y 34, Chuburná de Hidalgo, 97205, Mérida, Yucatán, Mexico. mmarcela@cicy.mx. 4. Departamento de Microbiología Ambiental y Biotecnología, Universidad Autónoma de Campeche, Av. Agustín Melgar S/N Entre Calle 20 y Juan de la Barrera, Col. Buenavista, 24039, San Francisco de Campeche, Campeche, Mexico. mamreyes@uacam.mx.
Abstract
AIM: This study was designed to investigate if culturing nematophagous fungi (NF) in the presence of a Haemonchus contortus larva crude extract (HcCE) enhances the nematocidal activity of nematophagous fungi liquid culture filtrates (NFCF). MATERIALS AND METHODS: Four NF Arthrobotrys oligospora, A. musiformis, Duddingtonia flagrans and Clonostachys rosea were cultured in flasks (n = 5) containing Czapek-Dox broth medium (CDB) in the presence or absence of HcCE. NFCF recovered by filtration of each fungus (200 mg/mL) were assessed on H. contortus infective larvae (L3) using 96-well micro-titer plates (n = 4). Additionally, CDB and water were considered negative controls, while Ivermectin acted as a positive control. After 48 h confrontation, ten 10-μL aliquots of each well were deposited on slides and observed under the microscope (40 ×). Dead and alive larvae in the aliquots were quantified, and a mortality rate (MR) was estimated. RESULTS: The MR of the different NFCF was greatly enhanced by the presence of HcCE. The four NF incubated in the absence of HcCE showed low mortality percentages from 8.2 to 25.8%; in contrast, when the assessed NF growth in the presence of HcCE showed a lethal activity ranging from 66.8 to 80.5%. Only C. rosea showed a moderate increase in the presence of the elicitor (42.7%). CONCLUSION: This study shows evidence about the HcCE enhances the production of nematocidal activity in NFCF. Future studies should be performed to elucidate the compounds responsible of the nematocidal activity that could have important implications in the control of sheep haemonchosis.
AIM: This study was designed to investigate if culturing nematophagous fungi (NF) in the presence of a Haemonchus contortus larva crude extract (HcCE) enhances the nematocidal activity of nematophagous fungi liquid culture filtrates (NFCF). MATERIALS AND METHODS: Four NF Arthrobotrys oligospora, A. musiformis, Duddingtonia flagrans and Clonostachys rosea were cultured in flasks (n = 5) containing Czapek-Dox broth medium (CDB) in the presence or absence of HcCE. NFCF recovered by filtration of each fungus (200 mg/mL) were assessed on H. contortus infective larvae (L3) using 96-well micro-titer plates (n = 4). Additionally, CDB and water were considered negative controls, while Ivermectin acted as a positive control. After 48 h confrontation, ten 10-μL aliquots of each well were deposited on slides and observed under the microscope (40 ×). Dead and alive larvae in the aliquots were quantified, and a mortality rate (MR) was estimated. RESULTS: The MR of the different NFCF was greatly enhanced by the presence of HcCE. The four NF incubated in the absence of HcCE showed low mortality percentages from 8.2 to 25.8%; in contrast, when the assessed NF growth in the presence of HcCE showed a lethal activity ranging from 66.8 to 80.5%. Only C. rosea showed a moderate increase in the presence of the elicitor (42.7%). CONCLUSION: This study shows evidence about the HcCE enhances the production of nematocidal activity in NFCF. Future studies should be performed to elucidate the compounds responsible of the nematocidal activity that could have important implications in the control of sheep haemonchosis.
Authors: Ching-Ting Yang; Guillermo Vidal-Diez de Ulzurrun; A Pedro Gonçalves; Hung-Che Lin; Ching-Wen Chang; Tsung-Yu Huang; Sheng-An Chen; Cheng-Kuo Lai; Isheng J Tsai; Frank C Schroeder; Jason E Stajich; Yen-Ping Hsueh Journal: Proc Natl Acad Sci U S A Date: 2020-03-11 Impact factor: 11.205
Authors: J Charlier; L Rinaldi; V Musella; H W Ploeger; C Chartier; H Rose Vineer; B Hinney; G von Samson-Himmelstjerna; B Băcescu; M Mickiewicz; T L Mateus; M Martinez-Valladares; S Quealy; H Azaizeh; B Sekovska; H Akkari; S Petkevicius; L Hektoen; J Höglund; E R Morgan; D J Bartley; E Claerebout Journal: Prev Vet Med Date: 2020-07-26 Impact factor: 2.670
Authors: L Aguilar-Marcelino; M T Quintero-Martínez; P Mendoza de Gives; M E López-Arellano; E Liébano-Hernández; G Torres-Hernández; J M González-Camacho; I Cid del Prado Journal: J Helminthol Date: 2012-10-10 Impact factor: 2.170
Authors: M A Zarza-Albarrán; A Olmedo-Juárez; R Rojo-Rubio; P Mendoza-de Gives; M González-Cortazar; D Tapia-Maruri; J Mondragón-Ancelmo; C García-Hernández; Ever A Blé-González; A Zamilpa Journal: J Ethnopharmacol Date: 2019-11-15 Impact factor: 4.360