D Chen1, Z Chen1, Z Wang1, Y Yang1, Y Jiang1, C Hu1. 1. Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Abstract
OBJECTIVE: To investigate the anti-tumor effect of BSA@CuS-PEG nanocomposites on tongue squamous cell carcinoma. METHODS: Transmission electron microscopy, dynamic light scattering, Zeta potential and ultraviolet absorption spectroscopy were used to characterize the synthesized BSA@CuS-PEG nanocomposite, whose photothermal properties was assessed with near infrared Ⅱ region excitation light (1064 nm). The cytotoxicity of the nanocomposite in Cal27 and SCC9 cells was evaluated using CCK-8 assay, and its effect on cell cycle distribution was analyzed using flow cytometry. The in vivo antitumor effect of BSA@CuS-PEG was investigated in a Balb/c mouse model bearing subcutaneous Cal27 tumor xenograft. RESULTS: The synthesized BSA@CuS-PEG nanocomposite showed a temperature variation (ΔT) of about 30 ℃ under near-infrared (NIR) irradiation (0.5 W/cm2), suggesting its excellent photothermal sensitivity. CCK-8 assay showed that BSA@CuS-PEG had no significant toxicity to tumor cells, but upon NIR irradiation, the nanocomposite produced a significant stronger inhibitory effect on Cal27 and SCC9 cells than free nanocomposites (P < 0.001). Cell cycle analysis showed that compared with free nanocomposites, BSA@CuS-PEG plus NIR irradiation caused more obvious cell cycle arrest at G2/M phase in tongue cancer cells (P < 0.001). In the tumor-bearing mice, BSA@CuS-PEG combined with NIR irradiation produced a significant anti-tumor effect as compared with saline treatment plus NIR irradiation (P < 0.001). CONCLUSION: The BSA@CuS-PEG nanocomposite shows prominent photothermal properties and good anti-tumor effects both in vivo and in vitro, and thus provides a promising method for non-invasive early diagnosis and non-surgical treatment of primary tongue cancer.
OBJECTIVE: To investigate the anti-tumor effect of BSA@CuS-PEG nanocomposites on tongue squamous cell carcinoma. METHODS: Transmission electron microscopy, dynamic light scattering, Zeta potential and ultraviolet absorption spectroscopy were used to characterize the synthesized BSA@CuS-PEG nanocomposite, whose photothermal properties was assessed with near infrared Ⅱ region excitation light (1064 nm). The cytotoxicity of the nanocomposite in Cal27 and SCC9 cells was evaluated using CCK-8 assay, and its effect on cell cycle distribution was analyzed using flow cytometry. The in vivo antitumor effect of BSA@CuS-PEG was investigated in a Balb/c mouse model bearing subcutaneous Cal27 tumor xenograft. RESULTS: The synthesized BSA@CuS-PEG nanocomposite showed a temperature variation (ΔT) of about 30 ℃ under near-infrared (NIR) irradiation (0.5 W/cm2), suggesting its excellent photothermal sensitivity. CCK-8 assay showed that BSA@CuS-PEG had no significant toxicity to tumor cells, but upon NIR irradiation, the nanocomposite produced a significant stronger inhibitory effect on Cal27 and SCC9 cells than free nanocomposites (P < 0.001). Cell cycle analysis showed that compared with free nanocomposites, BSA@CuS-PEG plus NIR irradiation caused more obvious cell cycle arrest at G2/M phase in tongue cancer cells (P < 0.001). In the tumor-bearing mice, BSA@CuS-PEG combined with NIR irradiation produced a significant anti-tumor effect as compared with saline treatment plus NIR irradiation (P < 0.001). CONCLUSION: The BSA@CuS-PEG nanocomposite shows prominent photothermal properties and good anti-tumor effects both in vivo and in vitro, and thus provides a promising method for non-invasive early diagnosis and non-surgical treatment of primary tongue cancer.
Entities:
Keywords:
copper sulfide; nanocomposites; photothermal therapy; tongue cancer
Authors: S Yanamoto; S Yamada; H Takahashi; I Yoshitomi; G Kawasaki; H Ikeda; T Minamizato; T Shiraishi; S Fujita; T Ikeda; I Asahina; M Umeda Journal: Int J Oral Maxillofac Surg Date: 2012-08-14 Impact factor: 2.789
Authors: A Barbato; C Panizzolo; M Gheno; L Sainati; E Favero; D Faggian; F Giusti; L Pesscolderungg; M La Rosa Journal: Pediatr Allergy Immunol Date: 2001-04 Impact factor: 6.377
Authors: Uma Prabhakar; Hiroshi Maeda; Rakesh K Jain; Eva M Sevick-Muraca; William Zamboni; Omid C Farokhzad; Simon T Barry; Alberto Gabizon; Piotr Grodzinski; David C Blakey Journal: Cancer Res Date: 2013-02-19 Impact factor: 12.701
Authors: Austin C V Doughty; Ashley R Hoover; Elivia Layton; Cynthia K Murray; Eric W Howard; Wei R Chen Journal: Materials (Basel) Date: 2019-03-07 Impact factor: 3.623
Authors: Alberto Curcio; Amanda K A Silva; Sonia Cabana; Ana Espinosa; Benoit Baptiste; Nicolas Menguy; Claire Wilhelm; Ali Abou-Hassan Journal: Theranostics Date: 2019-02-12 Impact factor: 11.556