Literature DB >> 3500750

T-lymphocyte colony formation of murine thymocytes in agar contained in glass capillaries.

H P Matthiessen1, C Echarti, J Gerber, H R Maurer.   

Abstract

Optimal growth conditions are presented for a new colony test with mouse thymocytes in agar contained in glass capillaries. The kinetics of colony growth and the dependence from the PHA-, I1-2-, agar- and 2-mercaptoethanol concentration are shown. The colony forming cells are identified as T-lymphocytes by usual morphology and by an indirect immunoperoxidase method using mouse anti-Thy 1.2 antibodies.

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Year:  1987        PMID： 3500750     DOI： 10.1007/BF00320224

Source DB:  PubMed          Journal:  Blut        ISSN： 0006-5242


  6 in total

1.  Development of colonies in vitro of mitogen-stimulated mouse T lymphocytes.

Authors:  B Sredni; Y Kalechman; H Michlin; L A Rozenszajn
Journal:  Nature       Date:  1976-01-15       Impact factor: 49.962

2.  In vitro culture of lymphocyte colonies in agar capillary tubes after PHA-stimulation.

Authors:  H R Maurer; R Maschler; R Dietrich; B Goebel
Journal:  J Immunol Methods       Date:  1977       Impact factor: 2.303

3.  Characterization of T lymphocyte colony-forming cells in the mouse. The colony-forming cell is confined to Lyt-1,2,3+ cell subsets in the thymus and the lymph nodes.

Authors:  C Röpke
Journal:  J Immunol       Date:  1984-04       Impact factor: 5.422

4.  T-lymphocyte colony formation of murine spleen cells in a one-stage micro agar culture.

Authors:  A J Ulmer; H D Flad; H G Opitz
Journal:  J Immunol Methods       Date:  1981       Impact factor: 2.303

5.  Automated scanning of bone marrow cell colonies growing in agar-containing glass capillaries.

Authors:  H R Maurer; R Henry
Journal:  Exp Cell Res       Date:  1976-12       Impact factor: 3.905

6.  Identification of Hodgkin and Sternberg-reed cells as a unique cell type derived from a newly-detected small-cell population.

Authors:  H Stein; J Gerdes; U Schwab; H Lemke; D Y Mason; A Ziegler; W Schienle; V Diehl
Journal:  Int J Cancer       Date:  1982-10-15       Impact factor: 7.396
  6 in total

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