| Literature DB >> 34997815 |
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Abstract
Entities:
Year: 2022 PMID: 34997815 PMCID: PMC8742226 DOI: 10.1111/jcmm.17075
Source DB: PubMed Journal: J Cell Mol Med ISSN: 1582-1838 Impact factor: 5.310
FIGURE 7TXN2 depletion and HP overexpression enhanced ferroptosis in lung cancer cell lines A549 and NCI‐H1299. (A) The efficiency of transfected cells was analysed via Western blot assays. (B) qPCR showed TXN2 and HP expression with erastin (10 µM) or RSL (1 µM) treatment for 24 h. (C) Cell viability assays for the cells with erastin (10 µM) or RSL (1 µM) treatment for 24 h. The iron concentrations (D), MDA concentrations (E), the relative ROS levels (F), the GSH concentrations (G) of the cells under the treatment of erastin (10 µM) or RSL (1 µM) for 24 h. H, Analysis of C11‐BODIPY581/591 fluorescence (scale bar =10 μm). (I) The alterations of mitochondrial ultrastructure of the indicated cells and white arrows indicated mitochondria (scale bar =500 nm). NC, negative control; EV, empty vector; OE, overexpressing; *p < .05, **p < .01