Literature DB >> 3499139

The mechanism of action of GTP on Ca2+ efflux from rat liver microsomal vesicles.

A P Dawson1, G Hills, J G Comerford.   

Abstract

1. Guanosine 5'-[gamma-thio]triphosphate (GTP[S]), if added before GTP, blocks both Ca2+ efflux promoted by GTP and the effect of GTP on enhancement of inositol 1,4,5-triphosphate (IP3)-promoted Ca2+ release from preloaded microsomal vesicles. If, however, GTP[S] is added after GTP, it does not reverse the Ca2+ efflux promoted by GTP, nor does it inhibit IP3-promoted Ca2+ release. 2. The effect of GTP in enhancing IP3-promoted Ca2+ release is maintained after washing the microsomal vesicles free of added GTP. After this treatment, enhancement of IP3-promoted Ca2+ efflux can be observed in the absence of poly(ethylene glycol). 3. Electron microscopy shows that during GTP treatment of microsomal vesicles there is rapid production of very large vesicular structures, apparently produced by fusion of smaller vesicles. 4. Light-scattering changes are detectable during the fusion process. 5. Both Ca2+ efflux promoted by GTP and the enhancement of IP3-promoted Ca2+ release seen in the presence of GTP can probably be attributed to GTP-dependent vesicle fusion.

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Year:  1987        PMID： 3499139      PMCID： PMC1147957          DOI： 10.1042/bj2440087

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  22 in total

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Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
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3.  Rapid mobilization of Ca2+ from rat insulinoma microsomes by inositol-1,4,5-trisphosphate.

Authors:  M Prentki; T J Biden; D Janjic; R F Irvine; M J Berridge; C B Wollheim
Journal:  Nature       Date:  1984 Jun 7-13       Impact factor: 49.962

4.  Physiological concentrations of GTP stimulate fusion of the endoplasmic reticulum and the nuclear envelope.

Authors:  J Paiement
Journal:  Exp Cell Res       Date:  1984-04       Impact factor: 3.905

5.  Inositol (1,4,5)trisphosphate-promoted Ca2+ release from microsomal fractions of rat liver.

Authors:  A P Dawson; R F Irvine
Journal:  Biochem Biophys Res Commun       Date:  1984-05-16       Impact factor: 3.575

6.  The effect of inositol trisphosphate on Ca2+ fluxes in insulin-secreting tumor cells.

Authors:  S K Joseph; R J Williams; B E Corkey; F M Matschinsky; J R Williamson
Journal:  J Biol Chem       Date:  1984-11-10       Impact factor: 5.157

7.  Inositol 1,4,5-trisphosphate mobilizes intracellular Ca2+ from permeabilized insulin-secreting cells.

Authors:  T J Biden; M Prentki; R F Irvine; M J Berridge; C B Wollheim
Journal:  Biochem J       Date:  1984-10-15       Impact factor: 3.857

8.  myo-Inositol 1,4,5-trisphosphate. A second messenger for the hormonal mobilization of intracellular Ca2+ in liver.

Authors:  S K Joseph; A P Thomas; R J Williams; R F Irvine; J R Williamson
Journal:  J Biol Chem       Date:  1984-03-10       Impact factor: 5.157

9.  Ca2+ homeostasis in permeabilized human neutrophils. Characterization of Ca2+-sequestering pools and the action of inositol 1,4,5-triphosphate.

Authors:  M Prentki; C B Wollheim; P D Lew
Journal:  J Biol Chem       Date:  1984-11-25       Impact factor: 5.157

10.  Release of Ca2+ from a non-mitochondrial store site in peritoneal macrophages treated with saponin by inositol 1,4,5-trisphosphate.

Authors:  M Hirata; E Suematsu; T Hashimoto; T Hamachi; T Koga
Journal:  Biochem J       Date:  1984-10-01       Impact factor: 3.857
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  18 in total

1.  GTP-mediated Ca2+ release in rough endoplasmic reticulum. Correlation with a GTP-sensitive increase in membrane permeability.

Authors:  C V Nicchitta; S K Joseph; J R Williamson
Journal:  Biochem J       Date:  1987-12-15       Impact factor: 3.857

2.  Characterization of high-affinity ryanodine-binding sites of rat liver endoplasmic reticulum. Differences between liver and skeletal muscle.

Authors:  V Shoshan-Barmatz; T A Pressley; S Higham; N Kraus-Friedmann
Journal:  Biochem J       Date:  1991-05-15       Impact factor: 3.857

3.  The perturbation, by aluminium, of receptor-generated calcium transients in hepatocytes is not due to effects of Ins(1,4,5)P3-stimulated Ca2+ release or Ins(1,4,5)P3 metabolism by the 5-phosphatase and 3-kinase.

Authors:  S B Shears; A P Dawson; J W Loomis-Husselbee; P J Cullen
Journal:  Biochem J       Date:  1990-09-15       Impact factor: 3.857

4.  The effect of limited proteolysis on GTP-dependent Ca2+ efflux and GTP-dependent fusion in rat liver microsomal vesicles.

Authors:  J G Comerford; A P Dawson
Journal:  Biochem J       Date:  1989-03-15       Impact factor: 3.857

5.  Evidence that a low-molecular-mass GTP-binding protein is required for store-activated Ca2+ inflow in hepatocytes.

Authors:  K C Fernando; R B Gregory; F Katsis; B E Kemp; G J Barritt
Journal:  Biochem J       Date:  1997-12-01       Impact factor: 3.857

6.  The mechanism of action of GTP on Ca2+ efflux from rat liver microsomal vesicles. Measurement of vesicle fusion by fluorescence energy transfer.

Authors:  J G Comerford; A P Dawson
Journal:  Biochem J       Date:  1988-01-01       Impact factor: 3.857

7.  Calcium entry into the inositol 1,4,5-trisphosphate-releasable calcium pool is mediated by a GTP-regulatory mechanism.

Authors:  J M Mullaney; M Yu; T K Ghosh; D L Gill
Journal:  Proc Natl Acad Sci U S A       Date:  1988-04       Impact factor: 11.205

8.  Effects of CoA and acyl-CoA on Ca(2+)-permeability of endoplasmic-reticulum membranes from rat liver.

Authors:  G T Rich; J G Comerford; S Graham; A P Dawson
Journal:  Biochem J       Date:  1995-03-15       Impact factor: 3.857

9.  Multiple mechanisms by which protein kinase A potentiates inositol 1,4,5-trisphosphate-induced Ca2+ mobilization in permeabilized hepatocytes.

Authors:  G Hajnóczky; E Gao; T Nomura; J B Hoek; A P Thomas
Journal:  Biochem J       Date:  1993-07-15       Impact factor: 3.857

10.  Effects of CoA and acyl-CoAs on GTP-dependent Ca2+ release and vesicle fusion in rat liver microsomal vesicles.

Authors:  J G Comerford; A P Dawson
Journal:  Biochem J       Date:  1993-01-15       Impact factor: 3.857
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