Literature DB >> 34990949

UPLC-MS-MS-based lipidomics for the evaluation of changes in lipids during dry-cured mutton ham processing.

Xin Guo1, Di Shi1, Chengjiang Liu2, Yali Huang1, Qingling Wang1, Jingyun Wang1, Longying Pei3, Shiling Lu4.   

Abstract

Six key stages in the processing of dry-cured muton ham were selected, and changes in the main lipid metabolites as well as the major pathways involved were identified using a lipidic metabolomics approach based on UPLC-MS-MS. In total, 581 lipid metabolites from 22 subclasses were identified, including 521 significantly differential lipids (p < 0.05, VIP > 1). Glycerolipids (GL) were the most abundant lipids, followed by glycerophospholipids (GP), fatty acyls (FA), and sphingolipids (SL). PCA and OPLS-DA of metabolites showed that the quality of mutton ham changed the most during the P3 fermenting stage, including TG(18:1/18:2/18:3), PE(20:5/18:1), and TG(16:1/18:1/18:4) that were significantly downregulated, and CE(20:3), FFA(24:6), LPC(20:3/0:0), and FFA(18:4) that were significantly upregulated. Moreover, glycerophospholipid metabolism and sphingolipid metabolism were the key metabolic pathways involved in the processing of dry-cured mutton ham. Our results provide a basis for quality control and product improvement of dry-cured mutton ham.
Copyright © 2021 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  Lipid dynamic changes; Multiple reaction monitoring (MRM); Mutton ham; Processing; Quality control

Mesh:

Substances:

Year:  2021        PMID: 34990949     DOI: 10.1016/j.foodchem.2021.131977

Source DB:  PubMed          Journal:  Food Chem        ISSN: 0308-8146            Impact factor:   7.514


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