Henrique Jordem Venial1, Ana Montoya2, Rocío Checa2, Guadalupe Miró2, Gabriel José Silva Uzai3, Maria Aparecida da Silva4, Louisiane de Carvalho Nunes5, Renato Luiz Silveira6, Eulógio C Queiroz de Carvalho7. 1. Department of Veterinary Medicine, Federal University of Espírito Santo, Alto Universitário 16, Guararema, Alegre, Espírito Santo, 29500-000, Brazil. henriquevenial@hotmail.com. 2. Department of Animal Health, Veterinary Faculty, Complutense University of Madrid, Madrid, Spain. 3. Postgraduate in Animal Science, State University of Santa Cruz, Ilhéus, Bahia, Brazil. 4. Department of Biology Sciences, Federal University of Espírito Santo, Alegre, Espírito Santo, Brazil. 5. Department of Veterinary Medicine, Federal University of Espírito Santo, Alto Universitário 16, Guararema, Alegre, Espírito Santo, 29500-000, Brazil. 6. Department of Morphology, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil. 7. Laboratory of Animal Health, Department of Veterinary Medicine, State University of the North Fluminense Darcy Ribeiro, Campos dos Goytacazes, Rio de Janeiro, Brazil.
Abstract
PURPOSE: Leishmaniasis are infectious and zoonotic diseases and present in cutaneous and visceral forms. Cutaneous leishmaniasis is endemic and widely distributed throughout the state of Espírito Santo, Brazil. Several cases of cutaneous leishmaniasis in humans and dogs associated with Leishmania (Viannia) braziliensis have been reported in the state. This study aimed to investigate the occurrence of natural infection by Leishmania(Viannia) braziliensis in wild mammals found dead (by trampling or natural death) in the Sooretama Biological Reserve, Espírito Santo State. METHODS: From January 2018 to December 2019, 60 animals were collected. Of these, 47 animals from 12 different species were analyzed. The results were demonstrated using descriptive analysis of the observations to calculate the absolute and relative frequencies of the data. RESULTS: In the PCR, using specific primers for the genus Leishmania (D1, D2, and D3) and the species Leishmania (Viannia) braziliensis (ISVB/ISVC), 4 positive animals (8.5%) were detected: 1 Cuniculus paca (paca) (25%) and 3 Callithrix geoffroyi (white-faced marmoset) (25%). In the histopathological analysis, the parasitic amastigote form was not observed. CONCLUSIONS: The natural infection, detected by PCR, by Leishmania (Viannia) braziliensis in Cuniculus paca (paca) and Callithrix geoffroyi (white-faced marmoset) constitutes the first report of infection of this rodent and primate species in the literature. Despite the confirmation of Leishmania (Viannia) braziliensis infection in rodents and primates, the role of these species in the transmission of this zoonosis still needs further observational studies to identify their seasonal variation, transmissibility, infection stability, and the effects of a given parasite on the population and/or individual.
PURPOSE: Leishmaniasis are infectious and zoonotic diseases and present in cutaneous and visceral forms. Cutaneous leishmaniasis is endemic and widely distributed throughout the state of Espírito Santo, Brazil. Several cases of cutaneous leishmaniasis in humans and dogs associated with Leishmania (Viannia) braziliensis have been reported in the state. This study aimed to investigate the occurrence of natural infection by Leishmania(Viannia) braziliensis in wild mammals found dead (by trampling or natural death) in the Sooretama Biological Reserve, Espírito Santo State. METHODS: From January 2018 to December 2019, 60 animals were collected. Of these, 47 animals from 12 different species were analyzed. The results were demonstrated using descriptive analysis of the observations to calculate the absolute and relative frequencies of the data. RESULTS: In the PCR, using specific primers for the genus Leishmania (D1, D2, and D3) and the species Leishmania (Viannia) braziliensis (ISVB/ISVC), 4 positive animals (8.5%) were detected: 1 Cuniculus paca (paca) (25%) and 3 Callithrix geoffroyi (white-faced marmoset) (25%). In the histopathological analysis, the parasitic amastigote form was not observed. CONCLUSIONS: The natural infection, detected by PCR, by Leishmania (Viannia) braziliensis in Cuniculus paca (paca) and Callithrix geoffroyi (white-faced marmoset) constitutes the first report of infection of this rodent and primate species in the literature. Despite the confirmation of Leishmania (Viannia) braziliensis infection in rodents and primates, the role of these species in the transmission of this zoonosis still needs further observational studies to identify their seasonal variation, transmissibility, infection stability, and the effects of a given parasite on the population and/or individual.
Authors: Sinval P Brandão-Filho; Maria E Brito; Francisco G Carvalho; Edna A Ishikawa; Elisa Cupolillo; Lucile Floeter-Winter; Jeffrey J Shaw Journal: Trans R Soc Trop Med Hyg Date: 2003 May-Jun Impact factor: 2.184
Authors: Julius Lukes; Isabel L Mauricio; Gabriele Schönian; Jean-Claude Dujardin; Ketty Soteriadou; Jean-Pierre Dedet; Katrin Kuhls; K Wilber Quispe Tintaya; Milan Jirků; Eva Chocholová; Christos Haralambous; Francine Pratlong; Miroslav Oborník; Ales Horák; Francisco J Ayala; Michael A Miles Journal: Proc Natl Acad Sci U S A Date: 2007-05-21 Impact factor: 11.205
Authors: A Falqueto; P A Sessa; A L Ferreira; V P Vieira; C B Santos; J B Varejão; E Cupolillo; R Porrozzi; L E Carvalho-Paes; G Grimaldi Júnior Journal: Mem Inst Oswaldo Cruz Date: 2003-12 Impact factor: 2.743