| Literature DB >> 34986197 |
Janna Heide, Sophia Schulte, Matin Kohsar, Thomas Theo Brehm, Marissa Herrmann, Hendrik Karsten, Matthias Marget, Sven Peine, Alexandra M Johansson, Alessandro Sette, Marc Lütgehetmann, William W Kwok, John Sidney, Julian Schulze Zur Wiesch.
Abstract
[This corrects the article DOI: 10.1371/journal.ppat.1009842.].Entities:
Year: 2022 PMID: 34986197 PMCID: PMC8730411 DOI: 10.1371/journal.ppat.1010220
Source DB: PubMed Journal: PLoS Pathog ISSN: 1553-7366 Impact factor: 6.823
In vitro binding capacity of 14 SARS-CoV-2-specific peptides to 22 frequent HLA class II MHC molecules.
Binding capacities are expressed as IC50 nM values measured in classical in vitro binding assays based on inhibition of binding of a high affinity radiolabeled ligand to purified HLA molecules. High affinity binding is defined as IC50 < 1,000 nM and highlighted by bold font. For reasons of comprehensibility, values larger than 40,000 nM are indicated by a dash. The total number of alleles bound as well as the response frequency (RF) of the responding peptide is shown. Mem_P30 and Mem_P36, with the highest RFs, bound to 12 and 13 HLA molecules, respectively.
| Peptide | aa position | Sequence | Alleles bound | RF | DPB1* | DPA1*01:03 | DQA1*05:01 | DQA1*05:01 | DQA1*03:01 | DQA1*01:01 | DQA1*01:02 | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Env_11 | 46–60 | L | V | K | P | S | F | Y | V | Y | S | R | V | K | N | L | 13 | 36.1 |
|
| - | 5766 | - | - | 24387 |
| Mem_30 | 146–160 | R | G | H | L | R | I | A | G | H | H | L | G | R | C | D | 12 | 72.2 |
|
| - | 15141 | - | 37659 | - |
| Mem_34 | 166–180 | K | E | I | T | V | A | T | S | R | T | L | S | Y | Y | K | 13 | 41.7 | 1909 | 3337 | 13645 |
| 17391 | - | 1827 |
| Mem_36 | 176–190 | L | S | Y | Y | K | L | G | A | S | Q | R | V | A | G | D | 13 | 66.7 | 13822 | - | 22448 |
| - | 15193 |
|
| Ncl_11 | 51–65 | S | W | F | T | A | L | T | Q | H | G | K | E | D | L | K | 9 | 46.9 | - | 35760 | 30440 | 1304 | 19748 | - |
|
| Ncl_17 | 81–95 | D | D | Q | I | G | Y | Y | R | R | A | T | R | R | I | R | 7 | 56.3 | 34655 | - | - | 5067 | - | - | - |
| Ncl_18 | 86–100 | Y | Y | R | R | A | T | R | R | I | R | G | G | D | G | K | 6 | 56.3 | - | - | - | 6720 | - | - | - |
| Ncl_22 | 106–120 | P | R | W | Y | F | Y | Y | L | G | T | G | P | E | A | G | 5 | 34.4 | 10053 | - |
| 1278 |
| 2239 | 7882 |
| Ncl_25 | 121–135 | L | P | Y | G | A | N | K | D | G | I | I | W | V | A | T | 2 | 37.5 | 13013 | 20045 | 11706 | 14585 | 3795 | - |
|
| Ncl_26 | 126–140 | N | K | D | G | I | I | W | V | A | T | E | G | A | L | N | 9 | 50 | 4140 | 8348 |
| 4027 |
| - | 2643 |
| Ncl_44 | 216–230 | D | A | A | L | A | L | L | L | L | D | R | L | N | Q | L | 7 | 43.8 | 1162 | 2779 | 2487 | 22499 |
| 8458 | 2499 |
| Ncl_45 | 221–235 | L | L | L | L | D | R | L | N | Q | L | E | S | K | M | S | 9 | 43.8 |
|
| 3315 | - | 11904 | 23089 | 8081 |
| Ncl_54 | 266–280 | K | A | Y | N | V | T | Q | A | F | G | R | R | G | P | E | 9 | 43.8 | 8438 | 6056 | - |
| 33174 | - | 2936 |
| Ncl_70 | 346–360 | F | K | D | Q | V | I | L | L | N | K | H | I | D | A | Y | 13 | 53.1 |
|
| 30656 | 33658 | 9176 | 6010 | 8602 |