Literature DB >> 34986197

Correction: Broadly directed SARS-CoV-2-specific CD4+ T cell response includes frequently detected peptide specificities within the membrane and nucleoprotein in patients with acute and resolved COVID-19.

Janna Heide, Sophia Schulte, Matin Kohsar, Thomas Theo Brehm, Marissa Herrmann, Hendrik Karsten, Matthias Marget, Sven Peine, Alexandra M Johansson, Alessandro Sette, Marc Lütgehetmann, William W Kwok, John Sidney, Julian Schulze Zur Wiesch.   

Abstract

[This corrects the article DOI: 10.1371/journal.ppat.1009842.].

Entities:  

Year:  2022        PMID: 34986197      PMCID: PMC8730411          DOI: 10.1371/journal.ppat.1010220

Source DB:  PubMed          Journal:  PLoS Pathog        ISSN: 1553-7366            Impact factor:   6.823


In Table 6, the 4th column of HLA alleles, the allele for DQB1 is missing a number. The correct column heading is: DQA1*05:01/DQB1*03:01. Please see the correct Table 6 below.
Table 6

In vitro binding capacity of 14 SARS-CoV-2-specific peptides to 22 frequent HLA class II MHC molecules.

Binding capacities are expressed as IC50 nM values measured in classical in vitro binding assays based on inhibition of binding of a high affinity radiolabeled ligand to purified HLA molecules. High affinity binding is defined as IC50 < 1,000 nM and highlighted by bold font. For reasons of comprehensibility, values larger than 40,000 nM are indicated by a dash. The total number of alleles bound as well as the response frequency (RF) of the responding peptide is shown. Mem_P30 and Mem_P36, with the highest RFs, bound to 12 and 13 HLA molecules, respectively.

Peptideaa positionSequenceAlleles boundRFDPB1*02:01DPA1*01:03DPB1*04:01DQA1*05:01DQB1*02:01DQA1*05:01DQB1*03:01DQA1*03:01DQB1*03:02DQA1*01:01DQB1*05:01DQA1*01:02DQB1*06:02
Env_1146–60LVKPSFYVYSRVKNL1336.1 42 63 -5766--24387
Mem_30146–160RGHLRIAGHHLGRCD1272.2 569 445 -15141-37659-
Mem_34166–180KEITVATSRTLSYYK1341.71909333713645 649 17391-1827
Mem_36176–190LSYYKLGASQRVAGD1366.713822-22448 48 -15193 428
Ncl_1151–65SWFTALTQHGKEDLK946.9-3576030440130419748- 952
Ncl_1781–95DDQIGYYRRATRRIR756.334655--5067---
Ncl_1886–100YYRRATRRIRGGDGK656.3---6720---
Ncl_22106–120PRWYFYYLGTGPEAG534.410053- 79 1278 414 22397882
Ncl_25121–135LPYGANKDGIIWVAT237.5130132004511706145853795- 203
Ncl_26126–140NKDGIIWVATEGALN95041408348 422 4027 103 -2643
Ncl_44216–230DAALALLLLDRLNQL743.811622779248722499 860 84582499
Ncl_45221–235LLLLDRLNQLESKMS943.8 285 701 3315-11904230898081
Ncl_54266–280KAYNVTQAFGRRGPE943.884386056- 794 33174-2936
Ncl_70346–360FKDQVILLNKHIDAY1353.1 287 794 3065633658917660108602

In vitro binding capacity of 14 SARS-CoV-2-specific peptides to 22 frequent HLA class II MHC molecules.

Binding capacities are expressed as IC50 nM values measured in classical in vitro binding assays based on inhibition of binding of a high affinity radiolabeled ligand to purified HLA molecules. High affinity binding is defined as IC50 < 1,000 nM and highlighted by bold font. For reasons of comprehensibility, values larger than 40,000 nM are indicated by a dash. The total number of alleles bound as well as the response frequency (RF) of the responding peptide is shown. Mem_P30 and Mem_P36, with the highest RFs, bound to 12 and 13 HLA molecules, respectively.
  1 in total

1.  Broadly directed SARS-CoV-2-specific CD4+ T cell response includes frequently detected peptide specificities within the membrane and nucleoprotein in patients with acute and resolved COVID-19.

Authors:  Janna Heide; Sophia Schulte; Matin Kohsar; Thomas Theo Brehm; Marissa Herrmann; Hendrik Karsten; Matthias Marget; Sven Peine; Alexandra M Johansson; Alessandro Sette; Marc Lütgehetmann; William W Kwok; John Sidney; Julian Schulze Zur Wiesch
Journal:  PLoS Pathog       Date:  2021-09-16       Impact factor: 6.823

  1 in total

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