Literature DB >> 34985741

High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems.

Conary Meyer1, Chuqing Zhou1, Zecong Fang1,2,3, Marjorie L Longo4, Tingrui Pan1,2,3,5,6, Cheemeng Tan7.   

Abstract

Cell-free protein synthesis can enable the combinatorial screening of many different components and concentrations. However, manual pipetting methods are unfit to handle many cell-free reactions. Here, we describe a microfluidic method that can generate hundreds of unique submicroliter scale reactions. The method is coupled with a high yield cell-free system that can be applied for broad protein screening assays.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Automation; Cell-free protein synthesis; Droplet printing; High throughput; Low-volume liquid handling; Microfluidic adaptive printing; Protein screening

Mesh：

Year:  2022        PMID： 34985741     DOI： 10.1007/978-1-0716-1998-8_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  13 in total

1.  Microfluidic-Enabled Print-to-Screen Platform for High-Throughput Screening of Combinatorial Chemotherapy.

Authors:  Yuzhe Ding; Jiannan Li; Wenwu Xiao; Kai Xiao; Joyce Lee; Urvashi Bhardwaj; Zijie Zhu; Philip Digiglio; Gaomai Yang; Kit S Lam; Tingrui Pan
Journal:  Anal Chem       Date:  2015-09-29       Impact factor: 6.986

2.  Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system.

Authors:  Zachary Z Sun; Enoch Yeung; Clarmyra A Hayes; Vincent Noireaux; Richard M Murray
Journal:  ACS Synth Biol       Date:  2013-12-04       Impact factor: 5.110

3.  Multi-dimensional studies of synthetic genetic promoters enabled by microfluidic impact printing.

Authors:  Jinzhen Fan; Fernando Villarreal; Brent Weyers; Yunfeng Ding; Kuo Hao Tseng; Jiannan Li; Baoqing Li; Cheemeng Tan; Tingrui Pan
Journal:  Lab Chip       Date:  2017-06-27       Impact factor: 6.799

4.  Dotette: Programmable, high-precision, plug-and-play droplet pipetting.

Authors:  Jinzhen Fan; Yongfan Men; Kuo Hao Tseng; Yi Ding; Yunfeng Ding; Fernando Villarreal; Cheemeng Tan; Baoqing Li; Tingrui Pan
Journal:  Biomicrofluidics       Date:  2018-05-21       Impact factor: 2.800

5.  High-precision digital droplet pipetting enabled by a plug-and-play microfluidic pipetting chip.

Authors:  Yuxin Mao; Yang Pan; Xuan Li; Baoqing Li; Jiaru Chu; Tingrui Pan
Journal:  Lab Chip       Date:  2018-09-11       Impact factor: 6.799

6.  A Plug-and-Play, Drug-on-Pillar Platform for Combination Drug Screening Implemented by Microfluidic Adaptive Printing.

Authors:  Jiannan Li; Wen Tan; Wenwu Xiao; Randy P Carney; Yongfan Men; Yuanpei Li; Gerald Quon; Yousif Ajena; Kit S Lam; Tingrui Pan
Journal:  Anal Chem       Date:  2018-11-13       Impact factor: 6.986

7.  Microfluidic impact printer with interchangeable cartridges for versatile non-contact multiplexed micropatterning.

Authors:  Yuzhe Ding; Eric Huang; Kit S Lam; Tingrui Pan
Journal:  Lab Chip       Date:  2013-03-25       Impact factor: 6.799

8.  A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery.

Authors:  Ashty S Karim; Michael C Jewett
Journal:  Metab Eng       Date:  2016-03-17       Impact factor: 9.783

9.  Microfluidic Print-to-Synthesis Platform for Efficient Preparation and Screening of Combinatorial Peptide Microarrays.

Authors:  Jiannan Li; Randy P Carney; Ruiwu Liu; Jinzhen Fan; Siwei Zhao; Yan Chen; Kit S Lam; Tingrui Pan
Journal:  Anal Chem       Date:  2018-04-13       Impact factor: 6.986

10.  Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology.

Authors:  James Chappell; Kirsten Jensen; Paul S Freemont
Journal:  Nucleic Acids Res       Date:  2013-01-31       Impact factor: 16.971
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