Literature DB >> 34985691

Fluorescence-Based Biosensors for the Detection of the Unfolded Protein Response.

Heinrich Kroukamp1,2, Kai Peng1, Ian T Paulsen1,2, Riaan den Haan3.   

Abstract

The unfolded protein response (UPR) is a highly conserved protein quality control mechanism of eukaryotic cells. Aberrations in this response have been linked to several human diseases, including retinitis pigmentosa and several cancers, and have been shown to have a drastic impact on recombinant protein yields in fungal, insect, and mammalian cell lines. Here, we describe the use of in vivo biosensors to measure and characterize this dynamic cellular response, specifically for detecting the UPR induced by protein overproduction stress in the model cell factory Saccharomyces cerevisiae.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Biosensors; Protein folding capacity; Saccharomyces cerevisiae; Unfolded protein response

Mesh：

Substances：

Year:  2022        PMID： 34985691     DOI： 10.1007/978-1-0716-1732-8_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  13 in total

1.  BiP acts as a molecular ratchet during posttranslational transport of prepro-alpha factor across the ER membrane.

Authors:  K E Matlack; B Misselwitz; K Plath; T A Rapoport
Journal:  Cell       Date:  1999-05-28       Impact factor: 41.582

2.  Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation.

Authors:  K J Travers; C K Patil; L Wodicka; D J Lockhart; J S Weissman; P Walter
Journal:  Cell       Date:  2000-04-28       Impact factor: 41.582

Review 3.  The unfolded protein response: from stress pathway to homeostatic regulation.

Authors:  Peter Walter; David Ron
Journal:  Science       Date:  2011-11-25       Impact factor: 47.728

4.  Protein production and induction of the unfolded protein response in Trichoderma reesei strain Rut-C30 and its transformant expressing endoglucanase I with a hydrophobic tag.

Authors:  Anna Collén; Markku Saloheimo; Michael Bailey; Merja Penttilä; Tiina M Pakula
Journal:  Biotechnol Bioeng       Date:  2005-02-05       Impact factor: 4.530

5.  Yeast unfolded protein response pathway regulates expression of genes for anti-oxidative stress and for cell surface proteins.

Authors:  Yukio Kimata; Yuki Ishiwata-Kimata; Seiko Yamada; Kenji Kohno
Journal:  Genes Cells       Date:  2006-01       Impact factor: 1.891

Review 6.  Metabolic engineering of recombinant protein secretion by Saccharomyces cerevisiae.

Authors:  Jin Hou; Keith E J Tyo; Zihe Liu; Dina Petranovic; Jens Nielsen
Journal:  FEMS Yeast Res       Date:  2012-05-17       Impact factor: 2.796

Review 7.  Signalling pathways in the unfolded protein response: development from yeast to mammals.

Authors:  Kazutoshi Mori
Journal:  J Biochem       Date:  2009-10-27       Impact factor: 3.387

8.  Real-time redox measurements during endoplasmic reticulum stress reveal interlinked protein folding functions.

Authors:  Philip I Merksamer; Ala Trusina; Feroz R Papa
Journal:  Cell       Date:  2008-11-20       Impact factor: 41.582

9.  High level secretion of cellobiohydrolases by Saccharomyces cerevisiae.

Authors:  Marja Ilmén; Riaan den Haan; Elena Brevnova; John McBride; Erin Wiswall; Allan Froehlich; Anu Koivula; Sanni P Voutilainen; Matti Siika-Aho; Daniël C la Grange; Naomi Thorngren; Simon Ahlgren; Mark Mellon; Kristen Deleault; Vineet Rajgarhia; Willem H van Zyl; Merja Penttilä
Journal:  Biotechnol Biofuels       Date:  2011-09-12       Impact factor: 6.040

10.  The unfolded protein response regulates multiple aspects of secretory and membrane protein biogenesis and endoplasmic reticulum quality control.

Authors:  D T Ng; E D Spear; P Walter
Journal:  J Cell Biol       Date:  2000-07-10       Impact factor: 10.539
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