Natural Biosurfactant as Antimicrobial Agent: Strategy to Action Against Fungal and Bacterial Activities.

Natural surfactants have gained importance as the usage of synthetic surfactants shows economical aspects, health, and environmental effect. This study examined the anti-microbial activity of safflower seed waste (Ssw) isolated surfactant against dandruff-causing Malassezia furfur and skin diseases causing bacterial strains. Saponin was the major component and non-ionic surfactants derived from plants, which have a special molecular structure with hydrophilic glycoside backbone and lipophilic triterpene derivative. The antimicrobial activity of isolated surfactants was confirmed by the MIC and kill-time assays. Our results showed that the isolated saponin may interact with the cell wall and membrane first and destroy the cell wall and membranes, which finally results in bacterial death. Besides, isolated saponin penetrates the cytoplasmic membrane or enters inside the cell after the destruction of cell structure, and then inhibits the normal synthesis of DNA and proteins that are required for bacterial growth. These results suggested that the effects of the Ssw isolated saponin on the growth inhibition of selected bacterial strains may be at the molecular level rather than only physical damage. Extraction of Biosurfactant (saponin) from Safflower seed waste and its antimicrobial activity.