Heba Bassiony Ghanem1,2, Marwa Nagy Emam3, Ali Dareen Abdelaziz Mohammed4, Abd-Ellatif Rania Nagi2. 1. Clinical laboratory sciences Department, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia. 2. Medical Biochemistry Department, Faculty of Medicine, Tanta University, Tanta, Egypt. 3. Physiology Department, Faculty of Medicine, Tanta University, Tanta, Egypt. 4. Pathology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Abstract
BACKGROUND: Epilepsy is one of the most widespread neurological disease worldwide. Status epilepticus (SE) is a life-threatening neurologic disorder. Neuroprotective approaches are increasingly to discover a promising therapy to manage epileptic disorders. This study aimed to assess the impact of berberine on some epigenetic, transcription regulation & inflammatory biomarkers in a mice model of epilepsy. METHODS: This work was performed on; Group I: (control), Group II: berberine-treated control,Group III: epilepsy group, Group IV: berberine-treated epilepsy. Groups were subjected to assessment of Tumor growth factor-1β (TGF-1β), hypoxia inducible factor-1α (HIF-1α), brain derived neurotrophic factor (BDNF) levels, histone deacetylase (HDAC) activity & neuronal restrictive silencing factor (NRSF) gene expression. RESULTS: Study showed significant increase in levels of HIF-1α, TGF-1β, HDAC activity & NRSF gene expression in epilepsy group & decrease in these levels in berberine treated epilepsy group. Significant decrease in BDNF levels in epilepsy & elevation in them in berberine treated epilepsy group. CONCLUSION: Our study showed the anti-epileptic impact of berberine via its regulatory effect on some epigenetic, transcription factors & inflammatory biomarkers in a mice model of epilepsy.
BACKGROUND: Epilepsy is one of the most widespread neurological disease worldwide. Status epilepticus (SE) is a life-threatening neurologic disorder. Neuroprotective approaches are increasingly to discover a promising therapy to manage epileptic disorders. This study aimed to assess the impact of berberine on some epigenetic, transcription regulation & inflammatory biomarkers in a mice model of epilepsy. METHODS: This work was performed on; Group I: (control), Group II: berberine-treated control,Group III: epilepsy group, Group IV: berberine-treated epilepsy. Groups were subjected to assessment of Tumor growth factor-1β (TGF-1β), hypoxia inducible factor-1α (HIF-1α), brain derived neurotrophic factor (BDNF) levels, histone deacetylase (HDAC) activity & neuronal restrictive silencing factor (NRSF) gene expression. RESULTS: Study showed significant increase in levels of HIF-1α, TGF-1β, HDAC activity & NRSF gene expression in epilepsy group & decrease in these levels in berberine treated epilepsy group. Significant decrease in BDNF levels in epilepsy & elevation in them in berberine treated epilepsy group. CONCLUSION: Our study showed the anti-epileptic impact of berberine via its regulatory effect on some epigenetic, transcription factors & inflammatory biomarkers in a mice model of epilepsy.
Authors: Guy Bar-Klein; Luisa P Cacheaux; Lyn Kamintsky; Ofer Prager; Itai Weissberg; Karl Schoknecht; Paul Cheng; Soo Young Kim; Lydia Wood; Uwe Heinemann; Daniela Kaufer; Alon Friedman Journal: Ann Neurol Date: 2014-05-28 Impact factor: 10.422
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