Dilek Battal1, Ayça Aktaş Süküroğlu2, Fehmi Burak Alkaş3, İrfan Ünlüsayın4. 1. Mersin University Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Mersin, Turkey. 2. Gazi University Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Ankara, Turkey. 3. Near East University Faculty of Pharmacy, Department of Toxicology, Nicosia, Cyprus. 4. Acıbadem Lab Med Research and Development Laboratory, İstanbul, Turkey.
Abstract
OBJECTIVES: Dopamine (DA) is a prominent biochemically complex neurotransmitter and immunomodulator. The quantification of DA could contribute to a better understanding of how endocrine system, cardiovascular and renal functions are regulated. The study aims to develop a rapid, precise, and extremely sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for routine clinical quantification of DA in urine. MATERIALS AND METHODS: Urine samples were extracted via one simple and rapid liquid-liquid extraction technique; then analyzed using a sensitive LC-MS/MS method developed by multiple reaction monitoring mode. RESULTS: DA and internal standard (IS) retention durations were found to be 2.28 min and 2.24 min, respectively. The mean extraction recovery of DA and DA-IS in urine was above 95.62%. DA calibration curve in urine was linear (r2≥0.998) ranging from 20 ng/mL to 1000 ng/mL. The maximum intra-day and inter-day precisions were 5.87 and 2.81, respectively and coefficients of variation were 10.55% and 7.57%, respectively. CONCLUSION: A rapid, precise, sensitive and quantitative LC-MS/MS detection of DA without the use of derivatization, evaporation, reconstitution and ion-pairing reagents has been developed with a simple and non-invasive sample technique for clinical laboratory applications, basic neuroscience research and drug development studies.
OBJECTIVES: Dopamine (DA) is a prominent biochemically complex neurotransmitter and immunomodulator. The quantification of DA could contribute to a better understanding of how endocrine system, cardiovascular and renal functions are regulated. The study aims to develop a rapid, precise, and extremely sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for routine clinical quantification of DA in urine. MATERIALS AND METHODS: Urine samples were extracted via one simple and rapid liquid-liquid extraction technique; then analyzed using a sensitive LC-MS/MS method developed by multiple reaction monitoring mode. RESULTS: DA and internal standard (IS) retention durations were found to be 2.28 min and 2.24 min, respectively. The mean extraction recovery of DA and DA-IS in urine was above 95.62%. DA calibration curve in urine was linear (r2≥0.998) ranging from 20 ng/mL to 1000 ng/mL. The maximum intra-day and inter-day precisions were 5.87 and 2.81, respectively and coefficients of variation were 10.55% and 7.57%, respectively. CONCLUSION: A rapid, precise, sensitive and quantitative LC-MS/MS detection of DA without the use of derivatization, evaporation, reconstitution and ion-pairing reagents has been developed with a simple and non-invasive sample technique for clinical laboratory applications, basic neuroscience research and drug development studies.
Authors: D Bose; A Durgbanshi; M E Capella-Peiró; M Gil-Agustí; J Esteve-Romero; S Carda-Broch; Adrià Martinavarro-Domínguez Journal: J Pharm Biomed Anal Date: 2004-10-29 Impact factor: 3.935
Authors: Wilhelmina H A de Jong; Marianne H L I Wilkens; Elisabeth G E de Vries; Ido P Kema Journal: Anal Bioanal Chem Date: 2010-02-07 Impact factor: 4.142