Literature DB >> 34972950

In Vitro and In Vivo Approaches to Study Kinetochore-Microtubule Attachments During Mitosis.

Sana Afreen1, Amit Rahi1, Adriana G Landeros1, Manas Chakraborty1, Richard J McKenney2, Dileep Varma3.   

Abstract

The separation of duplicated chromosomes during mitosis is a pivotal step in the process of cellular division. Therefore, the orchestrated events that take place to ensure proper attachment and stabilization of kMTs are keen areas of interest in the mitosis field. Here we describe the methods used to study kMT attachments via in vitro biochemical methods and in vivo cell biological approaches.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Cell cycle; Cell division; Cell synchronization; Chromosome; Kinetochores; Microscopy; Microtubules; Mitosis; Mitotic spindle; Photoactivation; TIR-FM; siRNA

Mesh：

Year:  2022        PMID： 34972950     DOI： 10.1007/978-1-0716-1904-9_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  Purification of brain tubulin through two cycles of polymerization-depolymerization in a high-molarity buffer.

Authors:  Mirco Castoldi; Andrei V Popov
Journal:  Protein Expr Purif       Date:  2003-11       Impact factor: 1.650

2.  The depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends.

Authors:  Jonne Helenius; Gary Brouhard; Yannis Kalaidzidis; Stefan Diez; Jonathon Howard
Journal:  Nature       Date:  2006-05-04       Impact factor: 49.962

3.  The conserved KMN network constitutes the core microtubule-binding site of the kinetochore.

Authors:  Iain M Cheeseman; Joshua S Chappie; Elizabeth M Wilson-Kubalek; Arshad Desai
Journal:  Cell       Date:  2006-12-01       Impact factor: 41.582

Review 4.  Molecular architecture of the kinetochore-microtubule interface.

Authors:  Iain M Cheeseman; Arshad Desai
Journal:  Nat Rev Mol Cell Biol       Date:  2008-01       Impact factor: 94.444

5.  Cooperative Accumulation of Dynein-Dynactin at Microtubule Minus-Ends Drives Microtubule Network Reorganization.

Authors:  Ruensern Tan; Peter J Foster; Daniel J Needleman; Richard J McKenney
Journal:  Dev Cell       Date:  2018-01-22       Impact factor: 12.270

Review 6.  The KMN protein network--chief conductors of the kinetochore orchestra.

Authors:  Dileep Varma; E D Salmon
Journal:  J Cell Sci       Date:  2013-02-15       Impact factor: 5.285

7.  Activation of cytoplasmic dynein motility by dynactin-cargo adapter complexes.

Authors:  Richard J McKenney; Walter Huynh; Marvin E Tanenbaum; Gira Bhabha; Ronald D Vale
Journal:  Science       Date:  2014-06-19       Impact factor: 47.728

8.  Kinetochore-microtubule attachment relies on the disordered N-terminal tail domain of Hec1.

Authors:  Geoffrey J Guimaraes; Yimin Dong; Bruce F McEwen; Jennifer G Deluca
Journal:  Curr Biol       Date:  2008-11-25       Impact factor: 10.834

9.  Cdt1 stabilizes kinetochore-microtubule attachments via an Aurora B kinase-dependent mechanism.

Authors:  Shivangi Agarwal; Kyle Paul Smith; Yizhuo Zhou; Aussie Suzuki; Richard J McKenney; Dileep Varma
Journal:  J Cell Biol       Date:  2018-08-28       Impact factor: 10.539

10.  Recruitment of the human Cdt1 replication licensing protein by the loop domain of Hec1 is required for stable kinetochore-microtubule attachment.

Authors:  Dileep Varma; Srikripa Chandrasekaran; Lynsie J R Sundin; Karen T Reidy; Xiaohu Wan; Dawn A D Chasse; Kathleen R Nevis; Jennifer G DeLuca; E D Salmon; Jeanette Gowen Cook
Journal:  Nat Cell Biol       Date:  2012-05-13       Impact factor: 28.824
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