Literature DB >> 3497206

An ELISA for anti-thymocyte autoantibody in NZB mice. Comparison with the cytotoxic assay.

M H Bocchieri.   

Abstract

An ELISA is described for the detection of anti-thymocyte antibody found in the serum of NZB autoimmune mice. This assay avoids the disadvantages of previous assay techniques which require carefully selected complement sources and/or radioactive labelled target cells. Thymocytes from a variety of strains of mice can be used as the antigenic targets, and the plates stored as long as 1 month. The lymphoma line EL4 can be used as the antigenic target in this assay, but it not as sensitive to most serum pools as thymocytes.

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Year:  1987        PMID: 3497206     DOI: 10.1016/0022-1759(87)90156-6

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  1 in total

1.  Autoantibodies to T and B cell lines detected in serum samples from patients with systemic lupus erythematosus with lymphopenia and hypocomplementaemia.

Authors:  M Noguchi; M Iwamori; T Hirano; S Kobayashi; H Hashimoto; S Hirose; Y Nagai
Journal:  Ann Rheum Dis       Date:  1992-06       Impact factor: 19.103

  1 in total

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