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Literature DB >> 3497092

Autoantibodies to purified nuclear proteins related to DNA metabolism during ageing and in SLE patients.

G C Astaldi Ricotti, M Pazzaglia, A M Martelli, A Cerino, M Bestagno, A Caprelli, S Riva, M A Pedrini, A Facchini.

Abstract

In this study the specificity of circulating autoantibodies in ANA+ aged donors, ANA- donors and SLE patients was investigated by immunoblotting on total nuclear proteins and by ELISA on purified nuclear proteins, possibly related to DNA metabolism, such as DNA polymerase alpha, DNA-dependent ATPase, DNA Topoisomerase I, ssDBP, hnRNP, HMG and histones. Immunoblotting showed that sera from ANA+ aged donors present fewer antibodies to nuclear proteins, especially to those between 21,000 and 45,000, molecular weight (MW), than sera from SLE patients. When the specificity of antisera was further studied on purified nuclear proteins, it was found that the majority of sera from SLE patients react with most of the proteins tested, whereas sera from ANA+ aged donors mainly react with DNA polymerase alpha, DNA-dependent ATPase, DNA Topoisomerase I and histones. In addition, sera from a few ANA- donors also reacted with certain purified nuclear proteins in a statistically significant age-related manner.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1987 PMID： 3497092 PMCID： PMC1453402

Source DB: PubMed Journal: Immunology ISSN： 0019-2805 Impact factor: 7.397

22 in total

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Authors: G H Goodwin; E W Johns
Journal: Methods Cell Biol Date: 1977 Impact factor: 1.441

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Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

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Authors: W F Marzluff; E C Murphy; R C Huang
Journal: Biochemistry Date: 1973-08-28 Impact factor: 3.162

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Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

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Authors: T Makinodan; M M Kay
Journal: Adv Immunol Date: 1980 Impact factor: 3.543

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Authors: M Reichlin
Journal: Clin Exp Immunol Date: 1981-04 Impact factor: 4.330

7. Association of antibodies to ribonucleoprotein and Sm antigens with mixed connective-tissue disease, systematic lupus erythematosus and other rheumatic diseases.

Authors: G C Sharp; W S Irvin; C M May; H R Holman; F C McDuffie; E V Hess; F R Schmid
Journal: N Engl J Med Date: 1976-11-18 Impact factor: 91.245

Autoantibodies to purified nuclear proteins related to DNA metabolism during ageing and in SLE patients.

1. The isolation and purification of the high mobility group (HMG) nonhistone chromosomal proteins.

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

3. Transcription of ribonucleic acid in isolated mouse myeloma nuclei.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Review 5. Age influence on the immune system.

Review 6. Current perspectives on serological reactions in SLE patients.

7. Association of antibodies to ribonucleoprotein and Sm antigens with mixed connective-tissue disease, systematic lupus erythematosus and other rheumatic diseases.

8. Autoantibodies to nucleosomal proteins: antibodies to HMG-17 in autoimmune diseases.

9. Mammalian single-stranded DNA binding protein UP I is derived from the hnRNP core protein A1.

10. Total purification of a DNA-dependent ATPase and of a DNA-binding protein from human cells.

1. Lupus autoantibodies to native DNA preferentially bind DNA presented on PolIV.

2. Autoantibodies to DNA topoisomerase II in cryptogenic fibrosing alveolitis and connective tissue disease.

3. Antibodies to topoisomerase II in idiopathic pulmonary fibrosis.