Literature DB >> 3496969

Kinetics of ATP-dependent Ca2+ uptake by permeabilized rat enterocytes. Effects of inositol 1,4,5-trisphosphate.

E J van Corven, P M Verbost, M D de Jong, C H van Os.   

Abstract

Isolated rat enterocytes were permeabilized by saponin treatment. 45Ca2+ was accumulated by these cells when provided with ATP in a medium containing Ca2+ ligands. The use of oxalate, vanadate and mitochondrial inhibitors indicated that both non-mitochondrial and mitochondrial pools are involved. Kinetic analysis of non-mitochondrial Ca2+ uptake revealed a Km of 0.1 microM Ca2+ and a Vmax of 0.4 nmol Ca2+/mg protein X min for this Ca2+-pumping ATPase activity. Mitochondria started to take up Ca2+ between 0.2 and 0.3 microM free Ca2+ reaching maximal rates around 2 microM. At 1 microM free Ca2+ mitochondria accumulated 20 times more Ca2+ than the non-mitochondrial pool. Inositol 1,4,5-trisphosphate released 40% of the Ca2+ content of the non-mitochondrial pool. Half-maximal release was observed at 0.5 and 1.5 microM IP3 in duodenal and ileal cells respectively. These findings support the possibility that the phosphatidyl inositide metabolism plays a role in regulation of electrolyte transport in enterocytes.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3496969     DOI： 10.1016/0143-4160(87)90018-2

Source DB:  PubMed          Journal:  Cell Calcium        ISSN： 0143-4160            Impact factor:   6.817


  6 in total

1.  Actions of cadmium on basolateral plasma membrane proteins involved in calcium uptake by fish intestine.

Authors:  T J Schoenmakers; P H Klaren; G Flik; R A Lock; P K Pang; S E Bonga
Journal:  J Membr Biol       Date:  1992-05       Impact factor: 1.843

2.  Proceedings of the British Pharmacological Society. University of Manchester, 13-15 September 1989.

Authors: 
Journal:  Br J Pharmacol       Date:  1989-12       Impact factor: 8.739

3.  1D-myo-inositol 1,4,5-trisphosphate dephosphorylation by rat enterocytes involves an intracellular 5-phosphatase and non-specific phosphatase activity at the cell surface.

Authors:  C Rubiera; G Velasco; R H Michell; P S Lazo; S B Shears
Journal:  Biochem J       Date:  1988-10-01       Impact factor: 3.857

4.  Polarized subcellular distribution of the 1-, 4- and 5-phosphatase activities that metabolize inositol 1,4,5-trisphosphate in intestinal epithelial cells.

Authors:  C Rubiera; P S Lazo; S B Shears
Journal:  Biochem J       Date:  1990-07-15       Impact factor: 3.857

5.  Effects of Zn(2+) on Ca (2+) uptake by mitochondria and endoplasmic reticulum in permeabilized tilapia gill cells.

Authors:  P M Verbost; M A Salah El-Deen; P Pelt; M M Bijvelds; S E Wendelaar Bonga
Journal:  Fish Physiol Biochem       Date:  1996-08       Impact factor: 2.794

6.  Intestinal secretagogues increase cytosolic free Ca2+ concentration and K+ conductance in a human intestinal epithelial cell line.

Authors:  T Yada; S Oiki; S Ueda; Y Okada
Journal:  J Membr Biol       Date:  1989-12       Impact factor: 1.843
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.