Literature DB >> 3496809

A fluorometric determination of N-methyl-4-phenylpyridinium ion, using high-performance liquid chromatography.

M Naoi, T Takahashi, T Nagatsu.   

Abstract

A simple and sensitive assay procedure for the quantitation of N-methyl-4-phenylpyridinium ion (MPP+), a neurotoxin, was devised using its fluorescence and high-performance liquid chromatography (HPLC). The fluorescence intensity of MPP+ was several thousand times more than that of N-methyl-1,2,3,6-tetrahydropyridine (MPTP), a metabolic precursor of MPP+. This method was found to be sensitive enough to measure less than 10 pmol MPP+ without using HPLC and 10 fmol using HPLC. The oxidation of MPTP by monoamine oxidase in human brain synaptosomal mitochondria was examined by this assay method. This fluorometric-HPLC method should have broad application in the study of the neurotoxin MPP+.

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Year:  1987        PMID： 3496809     DOI： 10.1016/0003-2697(87)90431-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  5 in total

1.  Susceptibility to a parkinsonian toxin varies during primate development.

Authors:  B A Morrow; R H Roth; D E Redmond; S Diano; J D Elsworth
Journal:  Exp Neurol       Date:  2012-02-14       Impact factor: 5.330

2.  Interaction of organic cation transporter 3 (SLC22A3) and amphetamine.

Authors:  Hao-Jie Zhu; David I Appel; Dirk Gründemann; John S Markowitz
Journal:  J Neurochem       Date:  2010-04-06       Impact factor: 5.372

3.  MPTP and DSP-4 susceptibility of substantia nigra and locus coeruleus catecholaminergic neurons in mice is independent of parkin activity.

Authors:  Bobby Thomas; Rainer von Coelln; Allen S Mandir; Daniel B Trinkaus; Mohamed H Farah; Kah Leong Lim; Noel Y Calingasan; M Flint Beal; Valina L Dawson; Ted M Dawson
Journal:  Neurobiol Dis       Date:  2007-01-25       Impact factor: 5.996

4.  Acute effects of 1-methyl-4-phenylpyridinium ion (MPP+) on dopamine and serotonin metabolism in rat striatum as assayed in vivo by a micro-dialysis technique.

Authors:  N Ozaki; D Nakahara; N Kaneda; K Kiuchi; T Okada; Y Kasahara; T Nagatsu
Journal:  J Neural Transm       Date:  1987       Impact factor: 3.575

5.  Attenuation of MPTP neurotoxicity by rolipram, a specific inhibitor of phosphodiesterase IV.

Authors:  Lichuan Yang; Noel Y Calingasan; Beverly J Lorenzo; M Flint Beal
Journal:  Exp Neurol       Date:  2007-02-27       Impact factor: 5.330
  5 in total

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