| Literature DB >> 3496396 |
E Kasp, J P Banga, E C Brown, J M Wicking, S Suleyman, B A Ellis, M D Sanders, D C Dumonde.
Abstract
This paper describes the use of phenyl-Sepharose CL-4B as a solid-phase hydrophobic adsorbent in the purification of S-antigen from protein extracts of bovine, porcine and human retina. Chromatographic conditions were ascertained whereby the majority of contaminating proteins were bound to the adsorbent leaving S-antigen in the liquid phase. In combination with size fractionation on Ultrogel AcA, the method conveniently yielded porcine and bovine S-antigen preparations up to 100% purity. Immunogenicity of purified S-antigens was verified by induction of experimental autoimmune uveoretinitis in albino Lewis rats. The method is preparative in scale, fast in performance and yields S-antigen in high purity and antigenic potency.Entities:
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Year: 1987 PMID： 3496396 DOI： 10.1016/0022-1759(87)90183-9
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303