Chlorpromazine, other amphiphilic cationic drugs and praziquantel: effects on carbohydrate metabolism of Schistosoma mansoni.

The effects of the amphiphilic cationic drugs chlorpromazine, imipramine, amitriptyline, propranolol and fluoxetine and praziquantel were investigated on glucose uptake and lactate excretion of Schistosoma mansoni. While praziquantel enhances glucose uptake and lactate excretion at a concentration of 10(-7) M, all the other drugs exert the same effects at concentrations above 10(-5) M. Generally, a constant molar ratio of 1:2 is found between glucose uptake and lactate excretion. Above 10(-5) M, praziquantel inhibits glucose uptake and lactate excretion. Similar effects are caused by amphiphilic cationic drugs at 10(-3) M. Pre-incubation of S. mansoni with 10(-5) M praziquantel completely abolish the stimulation of carbohydrate metabolism by serotonin and by 5 X 10(-5) M chlorpromazine or fluoxetine. The action of praziquantel on S. mansoni resembles that of amphiphilic cationic drugs with respect to their influence on carbohydrate metabolism. This, together with data obtained from electrophysiological and electron microscopic studies, provides evidence for the hypothesis that praziquantel exerts its effect by interacting with membrane structures.