Corrigendum: Heat Shock Protein HSP24 Is Involved in the BABA-Induced Resistance to Fungal Pathogen in Postharvest Grapes Underlying an NPR1-Dependent Manner.

[This corrects the article DOI: 10.3389/fpls.2021.646147.].

In the original article, there was an indeterminacy in Figure 5A as published. The figure depicted a small white dot of a single colony or a little colony cluster presented on the SD-T-L-H plate. We carelessly thought the white dot to be a bubble from the process of pouring the synthetic dropout medium into Petri dishes at first, because the single colony was extremely similar to a bubble. However, the presence of an actual single colony or colony cluster in SD-T-L-H plate might be caused by the self-activation of the “bait” pGBKT7 plasmid, thus leading to the “false positive” image in Y2H experiments with a very low probability (<5%). Of course, the result of His pull-down exhibited an obvious interaction between VvHSP24 and VvNPR1 in vitro.

Figure 5

VvHSP24 interacts with VvNPR1 in vivo and in vitro. (A) Yeast two-hybrid analysis of the physical interaction between VvHSP24 and VvNPR1. SD-T-L-H, SD/-Trp-Leu-His agar medium; SD-T-L-H-A, SD/-Trp-Leu-His-Ade agar medium. The right-angled triangles on the top of the gridded Petri dishes represent the absorbance of yeasts at 600 nm in a 10-fold dilution series, from 1 to 10−2 abs. (B) The GST-fused VvNPR1 protein (1 mL) was incubated with 1 mL of preimmobilized His-VvHSP24 protein in a total volume of 25 mL at 4°C for more than 8 h. The pulled down proteins (6 μL) were analyzed by western blotting with anti-His or anti-GST antibodies.

In past month, we re-conducted the Y2H with three replications following the method as described in the published M&M to completely confirm the interaction. Meanwhile, pull-down and co-IP were both done in this period. The obtained Y2H results showed that the colony clusters on SD-T-L-H and SD-T-L-H-A plates with or without X-α-gal were absolutely caused by the physical interaction between VvHSP24 and VvNPR1, but not the self-activation of the pGBKT7 vector, because no colony appeared among the negative controls (BD-VvHSP24 + AD and BD + AD-VvNPR1) in dropout plates. The pull-down and co-IP assays confirmed the interaction between VvHSP24 and VvNPR1 in vitro and in plant cells, which were consistent with the representative result of Y2H.

The correct Figure 5 appears below.

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

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