Literature DB >> 3495199

Purification of immunotoxins containing ricin A-chain and abrin A-chain using blue sepharose CL-6B.

P P Knowles, P E Thorpe.   

Abstract

We describe a method for separating antibody from immunotoxins by affinity chromatography on Cibacron blue F3GA coupled to Sepharose (Blue Sepharose). The antibody did not bind to the gel. The immunotoxins were bound by their ricin A-chain or abrin A-chain moiety and could be recovered in high yield and purity using mild elution conditions. The method is suitable for the large-scale purification of immunotoxins.

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Year:  1987        PMID： 3495199     DOI： 10.1016/0003-2697(87)90073-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  13 in total

Review 1.  Monoclonal antibodies. Future potential in cancer chemotherapy.

Authors:  C Kosmas; H Kalofonos; A A Epenetos
Journal:  Drugs       Date:  1989-11       Impact factor: 9.546

2.  Blocked and non-blocked ricin immunotoxins against the CD4 antigen exhibit higher cytotoxic potency than a ricin A chain immunotoxin potentiated with ricin B chain or with a ricin B chain immunotoxin.

Authors:  E J Wawrzynczak; G J Watson; A J Cumber; R V Henry; G D Parnell; E P Rieber; P E Thorpe
Journal:  Cancer Immunol Immunother       Date:  1991       Impact factor: 6.968

Review 3.  Immunotoxins for the treatment of B-cell lymphomas.

Authors:  M A Ghetie; V Ghetie; E S Vitetta
Journal:  Mol Med       Date:  1997-07       Impact factor: 6.354

4.  Rate of internalization of an immunotoxin correlates with cytotoxic activity against human tumor cells.

Authors:  U C Wargalla; R A Reisfeld
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

5.  An immunotoxin for the treatment of T-acute lymphoblastic leukemic meningitis: studies in rhesus monkeys.

Authors:  A A Hertler; D M Schlossman; M J Borowitz; D G Poplack; A E Frankel
Journal:  Cancer Immunol Immunother       Date:  1989       Impact factor: 6.968

Review 6.  Immunotoxins against solid tumors.

Authors:  R Pirker
Journal:  J Cancer Res Clin Oncol       Date:  1988       Impact factor: 4.553

7.  Comparative studies on kinetics of inhibition of protein synthesis in intact cells by ricin and a conjugate of ricin B-chain with momordin.

Authors:  S Sharma; S K Podder; A A Karande
Journal:  Mol Cell Biochem       Date:  1999-10       Impact factor: 3.396

8.  Human immunodeficiency virus-infected T cells and monocytes are killed by monoclonal human anti-gp41 antibodies coupled to ricin A chain.

Authors:  M A Till; S Zolla-Pazner; M K Gorny; J S Patton; J W Uhr; E S Vitetta
Journal:  Proc Natl Acad Sci U S A       Date:  1989-03       Impact factor: 11.205

9.  Comparison of blocked and non-blocked ricin-antibody immunotoxins against human gastric carcinoma and colorectal adenocarcinoma cell lines.

Authors:  L Cattel; L Delprino; P Brusa; F Dosio; P M Comoglio; M Prat
Journal:  Cancer Immunol Immunother       Date:  1988       Impact factor: 6.968

10.  Eradication of large solid tumors in mice with an immunotoxin directed against tumor vasculature.

Authors:  F J Burrows; P E Thorpe
Journal:  Proc Natl Acad Sci U S A       Date:  1993-10-01       Impact factor: 11.205
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