Literature DB >> 34950889

Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain.

Alessandro Venturino1, Sandra Siegert1.   

Abstract

Enzymatic digestion of the extracellular matrix with chondroitinase-ABC reinstates juvenile-like plasticity in the adult cortex as it also disassembles the perineuronal nets (PNNs). The disadvantage of the enzyme is that it must be applied intracerebrally and it degrades the ECM for several weeks. Here, we provide two minimally invasive and transient protocols for microglia-enabled PNN disassembly in mouse cortex: repeated treatment with ketamine-xylazine-acepromazine (KXA) anesthesia and 60-Hz light entrainment. We also discuss how to analyze PNNs within microglial endosomes-lysosomes. For complete details on the use and execution of this protocol, please refer to Venturino et al. (2021).
© 2021 The Author(s).

Entities:  

Keywords:  Bioinformatics; Immunology; Microscopy; Model Organisms; Neuroscience

Mesh：

Substances：

Year:  2021        PMID： 34950889      PMCID： PMC8672101          DOI： 10.1016/j.xpro.2021.101012

Source DB:  PubMed          Journal:  STAR Protoc        ISSN： 2666-1667


  23 in total

1.  Reactivation of ocular dominance plasticity in the adult visual cortex.

Authors:  Tommaso Pizzorusso; Paolo Medini; Nicoletta Berardi; Sabrina Chierzi; James W Fawcett; Lamberto Maffei
Journal:  Science       Date:  2002-11-08       Impact factor: 47.728

2.  Deletion of the Fractalkine Receptor, CX3CR1, Improves Endogenous Repair, Axon Sprouting, and Synaptogenesis after Spinal Cord Injury in Mice.

Authors:  Camila M Freria; Jodie C E Hall; Ping Wei; Zhen Guan; Dana M McTigue; Phillip G Popovich
Journal:  J Neurosci       Date:  2017-03-06       Impact factor: 6.167

3.  Ketamine: NMDA Receptors and Beyond.

Authors:  Charles F Zorumski; Yukitoshi Izumi; Steven Mennerick
Journal:  J Neurosci       Date:  2016-11-02       Impact factor: 6.167

4.  Electroencephalogram signatures of ketamine anesthesia-induced unconsciousness.

Authors:  Oluwaseun Akeju; Andrew H Song; Allison E Hamilos; Kara J Pavone; Francisco J Flores; Emery N Brown; Patrick L Purdon
Journal:  Clin Neurophysiol       Date:  2016-03-16       Impact factor: 3.708

5.  Accuracy and precision in quantitative fluorescence microscopy.

Authors:  Jennifer C Waters
Journal:  J Cell Biol       Date:  2009-06-29       Impact factor: 10.539

6.  Subcutaneous Compared with Intraperitoneal KetamineXylazine for Anesthesia of Mice.

Authors:  Maya Levin-Arama; Lital Abraham; Trevor Waner; Alon Harmelin; David M Steinberg; Tal Lahav; Mickey Harlev
Journal:  J Am Assoc Lab Anim Sci       Date:  2016-11       Impact factor: 1.232

7.  NMDA-receptor independent actions of ketamine: a new chapter in a story that's not so old.

Authors:  Nathan H Wray; Mark M Rasenick
Journal:  Neuropsychopharmacology       Date:  2019-01       Impact factor: 7.853

8.  Gamma Band Light Stimulation in Human Case Studies: Groundwork for Potential Alzheimer's Disease Treatment.

Authors:  Marggie Jones; Barry McDermott; Bárbara Luz Oliveira; Aoife O'Brien; Declan Coogan; Mark Lang; Niamh Moriarty; Eilis Dowd; Leo Quinlan; Brian Mc Ginley; Eoghan Dunne; David Newell; Emily Porter; Muhammad Adnan Elahi; Martin O' Halloran; Atif Shahzad
Journal:  J Alzheimers Dis       Date:  2019       Impact factor: 4.472

9.  V3D enables real-time 3D visualization and quantitative analysis of large-scale biological image data sets.

Authors:  Hanchuan Peng; Zongcai Ruan; Fuhui Long; Julie H Simpson; Eugene W Myers
Journal:  Nat Biotechnol       Date:  2010-03-14       Impact factor: 54.908

10.  Depletion of perineuronal nets enhances recognition memory and long-term depression in the perirhinal cortex.

Authors:  Carola Romberg; Sujeong Yang; Riccardo Melani; Melissa R Andrews; Alexa E Horner; Maria G Spillantini; Timothy J Bussey; James W Fawcett; Tommaso Pizzorusso; Lisa M Saksida
Journal:  J Neurosci       Date:  2013-04-17       Impact factor: 6.167
View more
  2 in total

1.  Update on Perineuronal Net Staining With Wisteria floribunda Agglutinin (WFA).

Authors:  Wolfgang Härtig; Anton Meinicke; Dominik Michalski; Stefan Schob; Carsten Jäger
Journal:  Front Integr Neurosci       Date:  2022-04-01

2.  A tool for mapping microglial morphology, morphOMICs, reveals brain-region and sex-dependent phenotypes.

Authors:  Gloria Colombo; Ryan John A Cubero; Lida Kanari; Alessandro Venturino; Rouven Schulz; Martina Scolamiero; Jens Agerberg; Hansruedi Mathys; Li-Huei Tsai; Wojciech Chachólski; Kathryn Hess; Sandra Siegert
Journal:  Nat Neurosci       Date:  2022-09-30       Impact factor: 28.771
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.