| Literature DB >> 3494788 |
A J Gearing, C R Bird, A Bristow, S Poole, R Thorpe.
Abstract
A subclone, NOB-1, of the mouse EL-4 line constitutively produces very little interleukin-2 but in response to interleukin-1 produces high concentrations of interleukin-2. Co-stimulation with mitogen, phorbol esters or calcium ionophores was not required. NOB-1 is not responsive to tumour necrosis factor alpha, tumour necrosis factor beta, interferon gamma and lipopolysaccharide. The NOB-1 line was used in conjunction with a CTLL line to detect less than 1 pg/ml interleukin-1. Rapid assay was performed by co-culturing the EL-4 cells with CTLL cells. By incorporating a pre-incubation step, followed by thorough washing of the EL-4 cells, responses to interleukin-1 were maintained, but interleukin-2 had no effect. The assay was used to detect interleukin-1 in serum samples and to evaluate neutralizing antisera to interleukin-1.Entities:
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Year: 1987 PMID： 3494788 DOI： 10.1016/0022-1759(87)90025-1
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303