Literature DB >> 3494768

Cellular localization of perforin 1 in murine cloned cytotoxic T lymphocytes.

P Groscurth, B Y Qiao, E R Podack, H Hengartner.   

Abstract

The localization of perforin 1 (P1) in cytotoxic cells was studied by immuno-electron microscopy by using a monospecific rabbit antiserum against highly purified mouse P1 and protein A gold as a second ligand. P1 was found in specific granules of cloned cytotoxic T lymphocytes (CTL). Within the granules, P1 antigen was localized in the fine granular matrix, whereas the vesicular compartment remained free of gold particles. The amount of P1 antigen detectable by immuno-electron microscopy varied between different CTL clones. CTL with NK-like activity had the highest level of P1 antigen. A cytotoxicity loss CTL mutant had no detectable P1 antigen, suggesting an important role of P1 during cell-mediated cytolysis. P1 antigen was undetectable also in bone marrow macrophages, indicating a different cytolytic mechanism of these cells.

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Year:  1987        PMID： 3494768

Source DB:  PubMed          Journal:  J Immunol        ISSN： 0022-1767            Impact factor:   5.422


  8 in total

1.  Ultrastructural changes during lysis of L929 target cells by class II-restricted influenza virus-specific murine cytotoxic T-lymphocyte clones.

Authors:  N Ono; K K Ko; Y Hosaka
Journal:  J Virol       Date:  1990-05       Impact factor: 5.103

Review 2.  Cytotoxic effector cells of the immune system.

Authors:  P Groscurth
Journal:  Anat Embryol (Berl)       Date:  1989

3.  Identification and sequencing of cDNA clones encoding the granule-associated serine proteases granzymes D, E, and F of cytolytic T lymphocytes.

Authors:  D Jenne; C Rey; J A Haefliger; B Y Qiao; P Groscurth; J Tschopp
Journal:  Proc Natl Acad Sci U S A       Date:  1988-07       Impact factor: 11.205

4.  Two proteins targeted to the same lytic granule compartment undergo very different posttranslational processing.

Authors:  J K Burkhardt; S Hester; Y Argon
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

5.  Expression of HRF20, a regulatory molecule of complement activation, on peripheral blood mononuclear cells.

Authors:  T Hideshima; N Okada; H Okada
Journal:  Immunology       Date:  1990-03       Impact factor: 7.397

6.  The lytic granules of natural killer cells are dual-function organelles combining secretory and pre-lysosomal compartments.

Authors:  J K Burkhardt; S Hester; C K Lapham; Y Argon
Journal:  J Cell Biol       Date:  1990-12       Impact factor: 10.539

7.  Cytotoxic T lymphocyte granules are secretory lysosomes, containing both perforin and granzymes.

Authors:  P J Peters; J Borst; V Oorschot; M Fukuda; O Krähenbühl; J Tschopp; J W Slot; H J Geuze
Journal:  J Exp Med       Date:  1991-05-01       Impact factor: 14.307

Review 8.  Human intraepithelial lymphocytes.

Authors:  A M Mowat
Journal:  Springer Semin Immunopathol       Date:  1990
  8 in total

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