Zanda Daneberga1,2, Miki Nakazawa-Miklasevica1, Egija Berga-Svitina1, Daiga Murmane3, Dana Isarova4, Liene Cupane5,6, Madara Masinska3, Inga Nartisa2, Andzela Lazdane2, Edvins Miklasevics1. 1. Institute of Oncology, Riga Stradins University, Riga, Latvia. 2. Laboratory, Children's Clinical University Hospital, Riga, Latvia. 3. Clinic of Medical Genetics and Prenatal Diagnosis, Children's Clinical University Hospital, Riga, Latvia. 4. Infection Prevention and Control Department, Children's Clinical University Hospital, Riga, Latvia. 5. Clinic of General Paediatrics, Children's Clinical University Hospital, Riga, Latvia. 6. Department of Paediatrics, Riga Stradins University, Riga, Latvia.
Abstract
Introduction: Alteration of human gut microbiota is described in a number of neuro-developmental and cognitive disorders including autistic spectrum disorder (ASD). Along with the changes in the gut microbiota, children with ASD are also reported to have changes in urinary organic acid spectra implying these metabolites as potential biomarkers for gastrointestinal dysbiosis.Aim: Identify urinary metabolites that would indicate specific changes in the gut microbiota and could be useful as biomarkers. Methods: The study group consisted of 44 children with ASD. Urinary organic acids spectra and composition of gut microbiota were analysed. Results: Any significant deviation in quantified metabolites compared to the reference values were not confirmed. The main variations were detected in concentration of p-cresol and 3-(3-hydroxyphenyl)-3-hydroxypropionic acid (HPHPA), but we cannot confirm the presence of HPHPA in urine as a biomarker for Clostridium sp. overgrowth in the gut. The acquired results indicate higher relative abundance of Firmicutes phylum alone may be attributed to increased concentration of p-cresol in urine. Decreased Bacteroidetes/Firmicutes ratio was found in the group with the presence of HPHPA in urine.Conclusions: Metabolites of human urine can be used as biomarkers for alterations of gut microbiota with caution, guided treatment should be administrated only based on gut microbiota analysis results or in combination with urinary organic acid results, but not solely based on organic acid biomarkers.
Introduction: Alteration of human gut microbiota is described in a number of neuro-developmental and cognitive disorders including autistic spectrum disorder (ASD). Along with the changes in the gut microbiota, children with ASD are also reported to have changes in urinary organic acid spectra implying these metabolites as potential biomarkers for gastrointestinal dysbiosis.Aim: Identify urinary metabolites that would indicate specific changes in the gut microbiota and could be useful as biomarkers. Methods: The study group consisted of 44 children with ASD. Urinary organic acids spectra and composition of gut microbiota were analysed. Results: Any significant deviation in quantified metabolites compared to the reference values were not confirmed. The main variations were detected in concentration of p-cresol and 3-(3-hydroxyphenyl)-3-hydroxypropionic acid (HPHPA), but we cannot confirm the presence of HPHPA in urine as a biomarker for Clostridium sp. overgrowth in the gut. The acquired results indicate higher relative abundance of Firmicutes phylum alone may be attributed to increased concentration of p-cresol in urine. Decreased Bacteroidetes/Firmicutes ratio was found in the group with the presence of HPHPA in urine.Conclusions: Metabolites of human urine can be used as biomarkers for alterations of gut microbiota with caution, guided treatment should be administrated only based on gut microbiota analysis results or in combination with urinary organic acid results, but not solely based on organic acid biomarkers.
Entities:
Keywords:
ASD; biomarkers; gut microbiota; urinary organic acids
Authors: Yuanpeng Zheng; Naika Z Prince; Lucia N Peralta Marzal; Sabbir Ahmed; Johan Garssen; Paula Perez Pardo; Aletta D Kraneveld Journal: Int J Mol Sci Date: 2022-09-20 Impact factor: 6.208