| Literature DB >> 34931802 |
Aziz Taghbalout1, Nathaniel Jillette1, Albert W Cheng1,2,3,4.
Abstract
Here we describe TALE.Sense, a versatile platform for sensing DNA sequences in live mammalian cells enabling programmable generation of a customable response that discerns cells containing specified sequence targets. The platform is based on the programmable DNA binding of transcription activator-like effector (TALE) coupled to conditional intein-reconstitution producing a trans-spliced ON-switch for a response circuit. TALE.Sense shows higher efficiency and dynamic range when compared to the reported zinc-finger based DNA-sensor in detecting same DNA sequences. Swapping transcriptional activation modules and introducing SunTag-based amplification loops to TALE.Sense circuits augment detection efficiency of the DNA sensor. The TALE.Sense platform shows versatility when applied to a range of target sites, indicating its suitability for applications to identify live cell variants with anticipated DNA sequences. TALE.Sense could be integrated with other cellular or synthetic circuits by using specified DNA sequences as control-switches, thus expanding the scope in connecting inducible modules for synthetic biology.Entities:
Keywords: DNA sensor; DNA sequence-targeting; TALE; biosensor; intein; trans-splicing; transcription activator-like effector
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Year: 2021 PMID： 34931802 PMCID： PMC9202581 DOI： 10.1021/acssynbio.1c00212
Source DB: PubMed Journal: ACS Synth Biol ISSN： 2161-5063 Impact factor: 5.249