Ahmed B M Mehany1, Amany Belal2, Aly Fahmy Mohamed3, Salwa Shaaban4,5, Ghada Abdelhamid6. 1. Genetic Engineering, Department of Zoology, Faculty of Science Al-Azhar University, Cairo, Egypt. 2. Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, Taif, Saudi Arabia. 3. Holding Company for Production of Vaccines and Biological Products (VACSERA), Agouza, Egypt. 4. Department of Microbiology& Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt. 5. Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia. 6. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Helwan University, Egypt.
Abstract
INTRODUCTION: Bladder cancer is still of unknown initiation and progression, it is difficult to treat the patient once bladder cancer have a distant metastasis. MATERIALS AND METHODS: In the present study, propolis extract was evaluated against bladder cancer cells (T24). Two independent pathways were investigated, apoptosis and angiogenesis, Bax, Bcl-2, P53, and caspase-3 for apoptosis, vascular endothelial growth factor receptor and protein kinase A as angiogenesis potential targets. OBJECTIVES: Molecular docking studies will be conducted for the major known constituents of Egyptian propolis into apoptotic and angiogenic protein targets, to give better insights to the possible binding mode and interactions and investigate the ability of propolis constituents to target both apoptotic and angiogenic pathways. RESULTS: Propolis showed anti-proliferative activity against T24 cancer cell line, the IC50 value was 6.36 µg/ml. Also significant effects of propolis on Bax, Bcl-2, P53, and caspase-3 were observed. DISCUSSION: These obtained results proved the ability of propolis to induce cell death. Also it has revealed noticeable effects on protein kinase A and vascular endothelial growth factor receptor. CONCLUSION: The obtained results can encourage us to say that propolis extract can induce a programmed cell death in human bladder cancer cells, and also affect angiogenesis.
INTRODUCTION: Bladder cancer is still of unknown initiation and progression, it is difficult to treat the patient once bladder cancer have a distant metastasis. MATERIALS AND METHODS: In the present study, propolis extract was evaluated against bladder cancer cells (T24). Two independent pathways were investigated, apoptosis and angiogenesis, Bax, Bcl-2, P53, and caspase-3 for apoptosis, vascular endothelial growth factor receptor and protein kinase A as angiogenesis potential targets. OBJECTIVES: Molecular docking studies will be conducted for the major known constituents of Egyptian propolis into apoptotic and angiogenic protein targets, to give better insights to the possible binding mode and interactions and investigate the ability of propolis constituents to target both apoptotic and angiogenic pathways. RESULTS: Propolis showed anti-proliferative activity against T24 cancer cell line, the IC50 value was 6.36 µg/ml. Also significant effects of propolis on Bax, Bcl-2, P53, and caspase-3 were observed. DISCUSSION: These obtained results proved the ability of propolis to induce cell death. Also it has revealed noticeable effects on protein kinase A and vascular endothelial growth factor receptor. CONCLUSION: The obtained results can encourage us to say that propolis extract can induce a programmed cell death in human bladder cancer cells, and also affect angiogenesis.
Entities:
Keywords:
Bladder cancer; T24; apoptosis, Bax, Bcl-2, P53, caspase-3, angiogenesis, VEGFR; human bladder cancer cell line; molecular docking; natural products; propolis; protein kinase A (PKA)
Authors: Amany Belal; Mohamed A Elanany; Eman Y Santali; Ahmed A Al-Karmalawy; Moustafa O Aboelez; Ali H Amin; Magda H Abdellattif; Ahmed B M Mehany; Hazem Elkady Journal: Molecules Date: 2022-06-02 Impact factor: 4.927
Authors: Amany Belal; Hazem Elkady; Ahmed A Al-Karmalawy; Ali H Amin; Mohammed M Ghoneim; Mohamed El-Sherbiny; Rasha Hamed Al-Serwi; Mohamed Attia Abdou; Mona H Ibrahim; Ahmed B M Mehany Journal: Molecules Date: 2022-08-30 Impact factor: 4.927