Literature DB >> 3492205

Stabilization and partial characterization of endothelium-derived relaxing factor from cultured bovine aortic endothelial cells.

J J Murray, I Fridovich, R G Makhoul, P O Hagen.   

Abstract

The extreme lability of the chemically undefined vasoregulatory mediator, endothelium-derived relaxing factor (EDRF), has been overcome. The activity of EDRF, from cultured bovine aortic endothelial cells stimulated with A23187, was stabilized by acidification. An additional action of EDRF, platelet disaggregation was used as a sensitive and convenient bioassay to monitor purification. EDRF appears to be a hydrophilic molecule, rapidly inactivated under alkaline conditions. However, activity is restored upon reacidification suggesting that this instability results from a readily reversible chemical process. The stabilization and partial purification of EDRF sets the stage for its further biochemical and chemical characterization.

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Year:  1986        PMID： 3492205     DOI： 10.1016/s0006-291x(86)80227-3

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  10 in total

1.  The inhibition of release of endothelium-derived relaxant factor by manoalide, a potent inhibitor of phospholipase A2.

Authors:  C J Long; H M Sarau; B A Berkowitz
Journal:  Br J Pharmacol       Date:  1987-12       Impact factor: 8.739

2.  Release of prostacyclin, endothelium-derived relaxing factor and endothelin by freshly harvested cells attached to microcarrier beads.

Authors:  S Kibira; R Dudek; K S Narayan; R J Bing
Journal:  Mol Cell Biochem       Date:  1991-11-13       Impact factor: 3.396

3.  Functional abnormalities of experimental autogenous vein graft neoendothelium.

Authors:  K S Cross; M N el-Sanadiki; J J Murray; E M Mikat; R L McCann; P O Hagen
Journal:  Ann Surg       Date:  1988-11       Impact factor: 12.969

4.  Endothelium-derived relaxant factor inhibits platelet activation.

Authors:  R Busse; A Lückhoff; E Bassenge
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1987-11       Impact factor: 3.000

5.  Platelet inhibition by endothelium-derived relaxing factor from the rabbit perfused aorta.

Authors:  H Bult; H R Fret; R M Van den Bossche; A G Herman
Journal:  Br J Pharmacol       Date:  1988-12       Impact factor: 8.739

6.  Paracrine modulation of androgen synthesis in rat leydig cells by nitric oxide.

Authors:  Ben A Weissman; Enmei Niu; Renshan Ge; Chantal M Sottas; Michael Holmes; James C Hutson; Matthew P Hardy
Journal:  J Androl       Date:  2005 May-Jun

7.  Stimulation of cyclic GMP production in cultured endothelial cells of the pig by bradykinin, adenosine diphosphate, calcium ionophore A23187 and nitric oxide.

Authors:  C Boulanger; V B Schini; S Moncada; P M Vanhoutte
Journal:  Br J Pharmacol       Date:  1990-09       Impact factor: 8.739

8.  Identification of arginine as a precursor of endothelium-derived relaxing factor.

Authors:  I Sakuma; D J Stuehr; S S Gross; C Nathan; R Levi
Journal:  Proc Natl Acad Sci U S A       Date:  1988-11       Impact factor: 11.205

9.  Reduction of intimal hyperplasia and enhanced reactivity of experimental vein bypass grafts with verapamil treatment.

Authors:  M N el-Sanadiki; K S Cross; J J Murray; R W Schuman; E Mikat; R L McCann; P O Hagen
Journal:  Ann Surg       Date:  1990-07       Impact factor: 12.969

10.  Evidence that inhibitory factor extracted from bovine retractor penis is nitrite, whose acid-activated derivative is stabilized nitric oxide.

Authors:  W Martin; J A Smith; M J Lewis; A H Henderson
Journal:  Br J Pharmacol       Date:  1988-03       Impact factor: 8.739
  10 in total

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