Literature DB >> 3491851

Antigen receptor-triggered secretion of a trypsin-type esterase from cytotoxic T lymphocytes.

H Takayama, G Trenn, W Humphrey, J A Bluestone, P A Henkart, M V Sitkovsky.   

Abstract

Exocytosis of cytolysin-containing granules from cytotoxic T lymphocytes (CTL) was studied with the use of granule enzyme (BLT esterase) as a convenient biochemical marker. Using cloned CTL, we demonstrate here that BLT esterase secretion into the supernatant is specifically triggered by antigen-bearing target cells and that this secretion is inhibited by soluble monoclonal antibodies against the antigen-specific T cell receptor (TcR). Immobilized anti-receptor antibodies induced efficient enzyme secretion in the absence of target cells, thus implying a direct involvement of TcR complex in triggering exocytosis of granules. These results support the role of the granule exocytosis in CTL functions and provide a quantitative and direct assay of a rapid CTL functional response to antigenic stimulation.

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Year:  1987        PMID： 3491851

Source DB:  PubMed          Journal:  J Immunol        ISSN： 0022-1767            Impact factor:   5.422


  21 in total

1.  Natural killer and lectin-dependent cytotoxic activities of Kurloff cells: target cell selectivity, conjugate formation, and Ca++ dependency.

Authors:  N Pouliot; K Maghni; F Blanchette; L Cironi; P Sirois; J Stankova; M Rola-Pleszczynski
Journal:  Inflammation       Date:  1996-12       Impact factor: 4.092

2.  The need for a new strategy for the treatment of multiple sclerosis.

Authors:  A N Davison
Journal:  J Neurol       Date:  1988-07       Impact factor: 4.849

3.  Cloning and chromosomal assignment of a human cDNA encoding a T cell- and natural killer cell-specific trypsin-like serine protease.

Authors:  H K Gershenfeld; R J Hershberger; T B Shows; I L Weissman
Journal:  Proc Natl Acad Sci U S A       Date:  1988-02       Impact factor: 11.205

4.  Two proteins targeted to the same lytic granule compartment undergo very different posttranslational processing.

Authors:  J K Burkhardt; S Hester; Y Argon
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

5.  The levels of soluble granzyme A and B are elevated in plasma and synovial fluid of patients with rheumatoid arthritis (RA).

Authors:  P P Tak; L Spaeny-Dekking; M C Kraan; F C Breedveld; C J Froelich; C E Hack
Journal:  Clin Exp Immunol       Date:  1999-05       Impact factor: 4.330

6.  A high-throughput phenotypic screen of cytotoxic T lymphocyte lytic granule exocytosis reveals candidate immunosuppressants.

Authors:  Ziyan Zhao; Mark K Haynes; Oleg Ursu; Bruce S Edwards; Larry A Sklar; Adam Zweifach
Journal:  J Biomol Screen       Date:  2014-11-07

7.  Raised granzyme B levels are associated with erosions in patients with early rheumatoid factor positive rheumatoid arthritis.

Authors:  R Goldbach-Mansky; S Suson; R Wesley; C E Hack; H S El-Gabalawy; P P Tak
Journal:  Ann Rheum Dis       Date:  2004-10-07       Impact factor: 19.103

8.  A null mutation in the perforin gene impairs cytolytic T lymphocyte- and natural killer cell-mediated cytotoxicity.

Authors:  B Lowin; F Beermann; A Schmidt; J Tschopp
Journal:  Proc Natl Acad Sci U S A       Date:  1994-11-22       Impact factor: 11.205

9.  Cutaneous leishmaniasis: co-ordinate expression of granzyme A and lymphokines by CD4+ T cells from susceptible mice.

Authors:  S Frischholz; M Röllinghoff; H Moll
Journal:  Immunology       Date:  1994-06       Impact factor: 7.397

10.  Two unique human leukemic T-cell lines endowed with a stable cytotoxic function and a different spectrum of target reactivity analysis and modulation of their lytic mechanisms.

Authors:  A Cesano; D Santoli
Journal:  In Vitro Cell Dev Biol       Date:  1992 Sep-Oct
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