Literature DB >> 34918295

Isolation of Fallopian Tube Epithelium for Assessment of Cilia Beating Frequency (CBF).

Angela Russo1, Joanna E Burdette2.   

Abstract

The fallopian tube epithelium (FTE) plays a critical role in reproduction and the genesis of ovarian cancer. The FTE columnar cells present with hair-like structures named "cilia" that are required for normal FTE function. Impairment of ciliary motion can lead to infertility, and it is influenced by hormonal signaling and endocrine disrupting compounds. Studying how cilia beating changes in response to these compounds is critical for understanding FTE physiology and pathology. In this protocol, we describe methods for isolating human fallopian tube epithelium, oviduct (murine equivalent of fallopian tube) epithelium, and ovaries. In addition, we describe methods for imaging and measuring cilia beating frequency using high-resolution time-lapse imaging.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Cilia beating frequency; Fallopian tube epithelium; Ovary; Oviduct; Ovulation

Mesh：

Year:  2022        PMID： 34918295      PMCID： PMC9088221          DOI： 10.1007/978-1-0716-1956-8_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  18 in total

Review 1.  Secreted proteins of the oviduct.

Authors:  W C Buhi; I M Alvarez; A J Kouba
Journal:  Cells Tissues Organs       Date:  2000       Impact factor: 2.481

Review 2.  Risk factors for ectopic pregnancy: a comprehensive analysis based on a large case-control, population-based study in France.

Authors:  Jean Bouyer; Joël Coste; Taraneh Shojaei; Jean-Luc Pouly; Hervé Fernandez; Laurent Gerbaud; Nadine Job-Spira
Journal:  Am J Epidemiol       Date:  2003-02-01       Impact factor: 4.897

3.  The effect of ovarian follicular fluid and peritoneal fluid on Fallopian tube ciliary beat frequency.

Authors:  R A Lyons; E Saridogan; O Djahanbakhch
Journal:  Hum Reprod       Date:  2005-09-19       Impact factor: 6.918

4.  Ciliary activity in the human oviduct.

Authors:  F N Critoph; K J Dennis
Journal:  Br J Obstet Gynaecol       Date:  1977-03

5.  Early transformative changes in normal ovarian surface epithelium induced by oxidative stress require Akt upregulation, DNA damage and epithelial-stromal interaction.

Authors:  Shelby M King; Suzanne M Quartuccio; Barbara C Vanderhyden; Joanna E Burdette
Journal:  Carcinogenesis       Date:  2013-01-08       Impact factor: 4.944

6.  Gonococcal infection of human fallopian tube mucosa in organ culture: relationship of mucosal tissue TNF-alpha concentration to sloughing of ciliated cells.

Authors:  Z A McGee; R L Jensen; C M Clemens; D Taylor-Robinson; A P Johnson; C R Gregg
Journal:  Sex Transm Dis       Date:  1999-03       Impact factor: 2.830

Review 7.  Somatic cell amplification of early pregnancy factors in the fallopian tube.

Authors:  R H F Hunter; N Einer-Jensen; T Greve
Journal:  Ital J Anat Embryol       Date:  2005

8.  Pathogenic mechanisms of Neisseria gonorrhoeae: observations on damage to human fallopian tubes in organ culture by gonococci of colony type 1 or type 4.

Authors:  Z A McGee; A P Johnson; D Taylor-Robinson
Journal:  J Infect Dis       Date:  1981-03       Impact factor: 5.226

9.  Both fallopian tube and ovarian surface epithelium are cells-of-origin for high-grade serous ovarian carcinoma.

Authors:  Shuang Zhang; Igor Dolgalev; Tao Zhang; Hao Ran; Douglas A Levine; Benjamin G Neel
Journal:  Nat Commun       Date:  2019-11-26       Impact factor: 14.919

10.  Spontaneous Transformation of Murine Oviductal Epithelial Cells: A Model System to Investigate the Onset of Fallopian-Derived Tumors.

Authors:  Michael P Endsley; Georgette Moyle-Heyrman; Subbulakshmi Karthikeyan; Daniel D Lantvit; David A Davis; Jian-Jun Wei; Joanna E Burdette
Journal:  Front Oncol       Date:  2015-07-17       Impact factor: 6.244
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