Ke Zeng1, Jia-Mo Zhang2, Xiao-Bin Li1, Sheng-Xian Peng3, Su-Chuan Zhang4, Wen-Xian Xie5, Chun-Fang Xi1, Cheng-Jian Cao6. 1. Department of Urology, First People's Hospital of Zigong City, Zigong, People's Republic of China. 2. Department of Urology, YongChuan Hospital Chongqing Medical University, Chongqing, People's Republic of China. 3. Scientific Research Department, First People's Hospital of Zigong City, Zigong, People's Republic of China. 4. Laboratory Department, First People's Hospital of Zigong City, Zigong, People's Republic of China. 5. Department of Basic Medicine, Sichuan Vocational College of Health and Rehabilitation, Zigong, People's Republic of China. 6. Zigong Academy of Medical Sciences, First People's Hospital of Zigong City, Zigong, People's Republic of China.
Abstract
OBJECTIVE: To analyze the bacterial biofilm (BF) formation in patients with malignancy undergoing double J stent indwelling and its influencing factors. METHODS: A total of 167 patients with malignant tumors who received double J stent indwelling in the hospital from January 2018 to January 2021 were included in the study. The urine and double J stent samples were collected for bacterial identification and observed for BF formation on the surface of the urinary catheter under a scanning electron microscope (SEM). Univariate and multivariate logistic regression analyses were used to analyze the influencing factors of BF. RESULTS: The BF formation rate was 34.73% (58/167). The BF formation rate of positive specimens cultured in urine and double J stent was significantly higher than that of negative ones (P<0.05). Staphylococcus was the main BF bacteria in double J stent and urine culture specimens, followed by Enterococcus, Pseudomonas, Enterobacter, and Acinetobacter. Compared with the non-BF group, the number of viable bacteria in the double J stent and urine and the catheterization time in the BF group rose markedly (P<0.05). Advanced age, chemotherapy, anemia, indwelling time ≥90d, and urinary tract infection were risk factors for BF formation in patients with malignancy undergoing double J stent indwelling (P<0.05). CONCLUSION: There is a high rate of BF formation in patients with malignancy undergoing double J stent indwelling, with Staphylococcus as the dominant species. Treatment requires enhanced urinary catheter management and nutritional status to inhibit BF formation and lower the rate of urinary catheter-related infections.
OBJECTIVE: To analyze the bacterial biofilm (BF) formation in patients with malignancy undergoing double J stent indwelling and its influencing factors. METHODS: A total of 167 patients with malignant tumors who received double J stent indwelling in the hospital from January 2018 to January 2021 were included in the study. The urine and double J stent samples were collected for bacterial identification and observed for BF formation on the surface of the urinary catheter under a scanning electron microscope (SEM). Univariate and multivariate logistic regression analyses were used to analyze the influencing factors of BF. RESULTS: The BF formation rate was 34.73% (58/167). The BF formation rate of positive specimens cultured in urine and double J stent was significantly higher than that of negative ones (P<0.05). Staphylococcus was the main BF bacteria in double J stent and urine culture specimens, followed by Enterococcus, Pseudomonas, Enterobacter, and Acinetobacter. Compared with the non-BF group, the number of viable bacteria in the double J stent and urine and the catheterization time in the BF group rose markedly (P<0.05). Advanced age, chemotherapy, anemia, indwelling time ≥90d, and urinary tract infection were risk factors for BF formation in patients with malignancy undergoing double J stent indwelling (P<0.05). CONCLUSION: There is a high rate of BF formation in patients with malignancy undergoing double J stent indwelling, with Staphylococcus as the dominant species. Treatment requires enhanced urinary catheter management and nutritional status to inhibit BF formation and lower the rate of urinary catheter-related infections.
Authors: Cortney E Heim; Megan E Bosch; Kelsey J Yamada; Amy L Aldrich; Sujata S Chaudhari; David Klinkebiel; Casey M Gries; Abdulelah A Alqarzaee; Yixuan Li; Vinai C Thomas; Edward Seto; Adam R Karpf; Tammy Kielian Journal: Nat Microbiol Date: 2020-07-13 Impact factor: 17.745