| Literature DB >> 34913113 |
Nishanthi Mathiyalagan1, Sebastian Dworkin2.
Abstract
Wholemount in-situ hybridization in zebrafish is a powerful technique for visualizing spatiotemporal gene expression during development. Here we describe a technique to detect endogenous mRNA expression in zebrafish that can be adapted to use on embryos from the single-cell stage until 5 days postfertilization.Entities:
Keywords: Craniofacial; Gene expression; In-situ hybridization; Neural crest; Riboprobe; Zebrafish
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Year: 2022 PMID: 34913113 DOI: 10.1007/978-1-0716-1847-9_2
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745