| Literature DB >> 34907763 |
Daliah Alves Coelho Trevisan1, Paula Aline Zanetti Campanerut-Sa1, Alex Fiori da Silva2, Andreia Farias Pereira Batista1, Flavio Augusto Vicente Seixas3, Rosane Marina Peralta4, Anacharis Babeto de Sa-Nakanishi4, Benicio Alves de Abreu Filho5, Miguel Machinski Junior5, Jane Martha Graton Mikcha1.
Abstract
Carvacrol presents action in Salmonella Typhimurium biofilms, however the antibiofilm mechanism of this compound has not been fully established yet. In the present study, the aim was to evaluate protein profile changes in S. Typhimurium biofilm treated with carvacrol. Proteomic analysis of treated versus untreated biofilm showed several changes in proteins involved with S. Typhimurium biofilm and antioxidant activity. The proteins DsbA (thiol: disulfide interchange protein DsbA), LuxS (S-ribosylhomocysteine lyase), DksA (RNA polymerase binding transcription factor DksA), and SODs (superoxide dismutases) A, B and C had their synthesis decreased after treatment with carvacrol. These proteins play a key role in S. Typhimurium biofilm formation, demonstrating the dynamic antibiofilm action of carvacrol. The differentially expressed proteins identified provide possible action targets for future studies in order to gain more insight into the mechanism of action of carvacrol on S. Typhimurium biofilm.Entities:
Keywords: Biofilms; Carvacrol; Proteome; Salmonella Typhimurium
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Year: 2020 PMID： 34907763 DOI： 10.32725/jab.2020.014
Source DB: PubMed Journal: J Appl Biomed ISSN： 1214-021X Impact factor: 1.797