Mekky M Abouzied1,2, Shaymaa M Mahmoud3, Ahmed Wahid4, Amr E Ahmed3, Ahmed M Okasha5, Hanan A Soliman6, Sultan S Al Thagfan7, Eman Z Attia8. 1. Taibah University, College of Pharmacy, Department of Pharmacology and Toxicology, Medina, Kingdom of Saudi Arabia. 2. Minia University, Faculty of Pharmacy, Department of Biochemistry, Minia, Egypt. 3. Beni-Suef University, Faculty of Postgraduate Studies for Advanced Sciences, Biotechnology Department, Beni-Suef, Egypt. 4. Alexandria University, Faculty of Pharmacy, Department of Pharmaceutical Biochemistry, Alexandria, Egypt. 5. Minia University, Faculty of Medicine, Department of Biochemistry, Minia, Egypt. 6. Beni-Suef University, Faculty of Science, Department of Biochemistry, Beni-Suef, Egypt. 7. Taibah University, College of Pharmacy, Department of Clinical and Hospital Pharmacy, Medina, Kingdom of Saudi Arabia. 8. Minia University, Faculty of Pharmacy, Department of Pharmacognosy, Minia, Egypt.
Abstract
BACKGROUND: The liver is the main metabolic organ involved in disposal and detoxification of various molecules. Plantago psyllium L. seed has been reported to exert positive effects in some pathological conditions. The current study aims to assess the hepatoprotective effect of Plantago psyllium L. seed extract against carbon tetrachloride-induced hepatotoxicity. METHODS: Male albino Wistar rats were randomly divided into five groups of 10 rats each. Hepatotoxicity was induced by orally administered carbon tetrachloride (CCl4) for nine weeks with or without the different treatments which were utilized daily for the whole nine weeks. Serum and tissue samples were then withdrawn and different liver biomarkers were investigated. RESULTS: Treatment of rats with Psyllium seed ethanolic extract significantly alleviated the toxic effects of CCl4. This was evidenced by its ability to restore liver biomarkers levels. Moreover, treatment with Psyllium seed extract normalized levels of oxidative biomarkers such as lipid peroxidation, hepatic content of reduced glutathione and catalase activity, as well as the expression level of the inflammatory marker TNF-α. Histopathological examination reflected the protective effect of the extract on liver architecture and confirmed the observed biochemical data. CONCLUSIONS: The presented data demonstrates a potential hepatoprotective effect of Psyllium seed extract compared to the standard hepatoprotective drug silymarin. This effect can be attributed to the antioxidant and anti-inflammatory effects of Psyllium extract.
BACKGROUND: The liver is the main metabolic organ involved in disposal and detoxification of various molecules. Plantago psyllium L. seed has been reported to exert positive effects in some pathological conditions. The current study aims to assess the hepatoprotective effect of Plantago psyllium L. seed extract against carbon tetrachloride-induced hepatotoxicity. METHODS: Male albino Wistar rats were randomly divided into five groups of 10 rats each. Hepatotoxicity was induced by orally administered carbon tetrachloride (CCl4) for nine weeks with or without the different treatments which were utilized daily for the whole nine weeks. Serum and tissue samples were then withdrawn and different liver biomarkers were investigated. RESULTS: Treatment of rats with Psyllium seed ethanolic extract significantly alleviated the toxic effects of CCl4. This was evidenced by its ability to restore liver biomarkers levels. Moreover, treatment with Psyllium seed extract normalized levels of oxidative biomarkers such as lipid peroxidation, hepatic content of reduced glutathione and catalase activity, as well as the expression level of the inflammatory marker TNF-α. Histopathological examination reflected the protective effect of the extract on liver architecture and confirmed the observed biochemical data. CONCLUSIONS: The presented data demonstrates a potential hepatoprotective effect of Psyllium seed extract compared to the standard hepatoprotective drug silymarin. This effect can be attributed to the antioxidant and anti-inflammatory effects of Psyllium extract.