Literature DB >> 34905172

Immunopeptidomic Analysis of the Phosphopeptidome Displayed by HLA Class I Molecules.

Miguel Marcilla1.   

Abstract

Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) is a powerful tool that allows the characterization of MHC-associated peptidomes. In addition to conventional nonmodified peptides, these complex mixtures also contain phosphorylated species, which may be of great interest for personalized cancer immunotherapy. Here, we provide a detailed protocol to identify phosphopeptides displayed by human HLA class I molecules consisting of four main steps: (1) immunopurification of MHC-I molecules, (2) phosphopeptide enrichment, (3) LC-MS/MS analysis, and (4) MS/MS ion search.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Affinity chromatography; HLA; IMAC; LC–MS/MS; MHC; MS/MS ion search; W6/32

Mesh：

Substances：

Year:  2022        PMID： 34905172     DOI： 10.1007/978-1-0716-1936-0_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  Phosphorylated peptides can be transported by TAP molecules, presented by class I MHC molecules, and recognized by phosphopeptide-specific CTL.

Authors:  M H Andersen; J E Bonfill; A Neisig; G Arsequell; I Sondergaard; G Valencia; J Neefjes; J Zeuthen; T Elliott; J S Haurum
Journal:  J Immunol       Date:  1999-10-01       Impact factor: 5.422

2.  The Choice of HLA-Associated Peptide Enrichment and Purification Strategy Affects Peptide Yields and Creates a Bias in Detected Sequence Repertoire.

Authors:  Annalisa Nicastri; Hanqing Liao; Julius Muller; Anthony W Purcell; Nicola Ternette
Journal:  Proteomics       Date:  2020-06-02       Impact factor: 3.984

3.  Phase I trial of a melanoma vaccine with gp100(280-288) peptide and tetanus helper peptide in adjuvant: immunologic and clinical outcomes.

Authors:  C L Slingluff; G Yamshchikov; P Neese; H Galavotti; S Eastham; V H Engelhard; D Kittlesen; D Deacon; S Hibbitts; W W Grosh; G Petroni; R Cohen; C Wiernasz; J W Patterson; B P Conway; W G Ross
Journal:  Clin Cancer Res       Date:  2001-10       Impact factor: 12.531

4.  ProteoWizard: open source software for rapid proteomics tools development.

Authors:  Darren Kessner; Matt Chambers; Robert Burke; David Agus; Parag Mallick
Journal:  Bioinformatics       Date:  2008-07-07       Impact factor: 6.937
  4 in total

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