Pseudaminobacter soli sp. nov., Isolated from Paddy Soil Contaminated with Heavy Metals.

A Gram stain-negative, aerobic, rod-shaped strain, designated HC19T, was isolated from heavy metals contaminated paddy soil. The 16S rRNA gene-based phylogenetic analysis indicated that strain HC19T belonged to the genus Pseudaminobacter, and shared 97.0% 16S rRNA gene sequence similarity with P. manganicus JH-7T, and less than 97% similarities with other type strains belonging to the genus. The major cellular fatty acids were C19:0 cyclo ω8c (55.0%) and C18: 1ω7c (18.7%). The major quinone was ubiquinone Q-10. The major polar lipids were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine. The average nucleotide identity and digital DNA-DNA hybridization values between the genomes of HC19T and P. manganicus JH-7T were 68.0% and 22%, respectively. The G+C content of the genomic DNA was 63.3 mol%. On the basis of phenotypic, chemotaxonomic, and genotypic data, strain HC19T is considered as a novel species in the genus Pseudaminobacter, for which the name Pseudaminobacter. soli sp. nov. is proposed. The type strain is HC19T (= KCTC 82870T = CCTCC AB 2021107T).