Literature DB >> 3488539

Complete nucleotide sequence of the nonstructural protein genes of Semliki Forest virus.

K Takkinen.   

Abstract

The nucleotide sequence coding for the nonstructural proteins of Semliki Forest virus has been determined from cDNA clones. The total length of this region is 7381 nucleotides, it contains an open reading frame starting at position 86 and ending at an UAA stop codon at position 7379-7381. This open reading frame codes for a 2431 amino acids long polyprotein, from which the individual nonstructural proteins are formed by proteolytic processing steps, so that nsPl is 537, nsP2 798, nsP3 482 and nsP4 614 amino acids. In the closely related Sindbis and Middelburg viruses there is an opal stop codon (UGA) between the genes for nsP3 and nsP4. Interestingly, no stop codon is found in frame in this region of the Semliki Forest virus 42S RNA. In other aspects the amino acid sequence homology between Sindbis, Middelburg and Semliki Forest virus nonstructural proteins is highly significant.

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Year:  1986        PMID： 3488539      PMCID： PMC311584          DOI： 10.1093/nar/14.14.5667

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  52 in total

1.  Semliki Forest virus intracellular RNA: properties of the multi-stranded RNA species and kinetics of positive and negative strand synthesis.

Authors:  C J Bruton; S I Kennedy
Journal:  J Gen Virol       Date:  1975-07       Impact factor: 3.891

2.  Proteins synthesized by Semliki Forest virus and its 16 temperature-sensitive mutants.

Authors:  S Keränen; L Kääriäinen
Journal:  J Virol       Date:  1975-08       Impact factor: 5.103

3.  Quantitation of Semlike Forest virus RNAs in infected cells using 32-P equilibrium labelling.

Authors:  K Tuomi; L Kädäridäinen; H Söderlund
Journal:  Nucleic Acids Res       Date:  1975-04       Impact factor: 16.971

4.  Polyadenylic acid sequences in the virus RNA species of cells infected with Semliki Forest Virus.

Authors:  J C Clegg; S I Kennedy
Journal:  J Gen Virol       Date:  1974-03       Impact factor: 3.891

5.  Calcium-dependent bacteriophage DNA infection.

Authors:  M Mandel; A Higa
Journal:  J Mol Biol       Date:  1970-10-14       Impact factor: 5.469

6.  New M13 vectors for cloning.

Authors:  J Messing
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

7.  Isolation and characterization of conditional-lethal mutants of Sindbis virus.

Authors:  B W Burge; E R Pfefferkorn
Journal:  Virology       Date:  1966-10       Impact factor: 3.616

8.  Tryptic peptide analysis on nonstructural and structural precursor proteins from Semliki Forest virus mutant-infected cells.

Authors:  B E Lachmi; N Glanville; S Keränen; L Lääriäinen
Journal:  J Virol       Date:  1975-12       Impact factor: 5.103

9.  Membrane-associated replication complex in arbovirus infection.

Authors:  R M Friedman; J G Levin; P M Grimley; I K Berezesky
Journal:  J Virol       Date:  1972-09       Impact factor: 5.103

10.  Specific membranous structures associated with the replication of group A arboviruses.

Authors:  P M Grimley; J G Levin; I K Berezesky; R M Friedman
Journal:  J Virol       Date:  1972-09       Impact factor: 5.103
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  66 in total

1.  Sequence requirements for Sindbis virus subgenomic mRNA promoter function in cultured cells.

Authors:  M M Wielgosz; R Raju; H V Huang
Journal:  J Virol       Date:  2001-04       Impact factor: 5.103

2.  Selection of RNA replicons capable of persistent noncytopathic replication in mammalian cells.

Authors:  I Frolov; E Agapov; T A Hoffman; B M Prágai; M Lippa; S Schlesinger; C M Rice
Journal:  J Virol       Date:  1999-05       Impact factor: 5.103

Review 3.  Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.

Authors:  H Beier; M Grimm
Journal:  Nucleic Acids Res       Date:  2001-12-01       Impact factor: 16.971

4.  Changes of the secondary structure of the 5' end of the Sindbis virus genome inhibit virus growth in mosquito cells and lead to accumulation of adaptive mutations.

Authors:  Rafik Fayzulin; Ilya Frolov
Journal:  J Virol       Date:  2004-05       Impact factor: 5.103

5.  Relationships among the positive strand and double-strand RNA viruses as viewed through their RNA-dependent RNA polymerases.

Authors:  J A Bruenn
Journal:  Nucleic Acids Res       Date:  1991-01-25       Impact factor: 16.971

6.  Mapping of RNA- temperature-sensitive mutants of Sindbis virus: complementation group F mutants have lesions in nsP4.

Authors:  Y S Hahn; A Grakoui; C M Rice; E G Strauss; J H Strauss
Journal:  J Virol       Date:  1989-03       Impact factor: 5.103

7.  Mutagenesis of the in-frame opal termination codon preceding nsP4 of Sindbis virus: studies of translational readthrough and its effect on virus replication.

Authors:  G P Li; C M Rice
Journal:  J Virol       Date:  1989-03       Impact factor: 5.103

8.  Alphavirus minus-strand RNA synthesis: identification of a role for Arg183 of the nsP4 polymerase.

Authors:  Cori L Fata; Stanley G Sawicki; Dorothea L Sawicki
Journal:  J Virol       Date:  2002-09       Impact factor: 5.103

9.  Modification of Asn374 of nsP1 suppresses a Sindbis virus nsP4 minus-strand polymerase mutant.

Authors:  Cori L Fata; Stanley G Sawicki; Dorothea L Sawicki
Journal:  J Virol       Date:  2002-09       Impact factor: 5.103

Review 10.  The alphaviruses: gene expression, replication, and evolution.

Authors:  J H Strauss; E G Strauss
Journal:  Microbiol Rev       Date:  1994-09
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