Literature DB >> 3487993

A semiautomated hemolysis microassay for membrane lytic proteins.

J D Young, L G Leong, M A DiNome, Z A Cohn.   

Abstract

A simple, rapid, semiautomated microassay for hemolysis using a microtiter plate spectrophotometric system is described. The assay relies on the differences in light scattering (turbidity) properties of intact and of lysed erythrocytes. Lysis of erythrocyte suspensions in 96-well plates is determined by absorbance at 690 nm. A linear correlation between the percentage of hemolysis and the turbidity decrease is observed, indicating that this assay may be used for both rapid screening and quantitation of the hemolytic activity. This assay allows screening of 300 samples in less than 6 min. Small samples derived from protein fractionation columns (HPLC, for example) can be rapidly screened. This assay has been used in the successful isolation of a cytolytic membrane-lytic protein from the granules of cloned cytotoxic T lymphocytes and NK cells.

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Year:  1986        PMID： 3487993     DOI： 10.1016/0003-2697(86)90042-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

1.  Structural and functional studies of truncated hemolysin A from Proteus mirabilis.

Authors:  Todd M Weaver; Joshua A Smith; Jason M Hocking; Lucas J Bailey; Grayson T Wawrzyn; David R Howard; Laura A Sikkink; Marina Ramirez-Alvarado; James R Thompson
Journal:  J Biol Chem       Date:  2009-06-03       Impact factor: 5.157

2.  Extracellular release of lymphocyte cytolytic pore-forming protein (perforin) after ionophore stimulation.

Authors:  J D Young; L G Leong; C C Liu; A Damiano; Z A Cohn
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205

3.  Protection by chlorpromazine, albumin and bivalent cations against haemolysis induced by melittin, [Ala-14]melittin and whole bee venom.

Authors:  S V Rudenko; E E Nipot
Journal:  Biochem J       Date:  1996-08-01       Impact factor: 3.857

4.  Sandwich enzyme-linked immunosorbent assay for Vibrio vulnificus hemolysin to detect V. vulnificus in environmental specimens.

Authors:  R W Parker; D H Lewis
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792

5.  Improved purification and characterization of hemolysin BL, a hemolytic dermonecrotic vascular permeability factor from Bacillus cereus.

Authors:  D J Beecher; A C Wong
Journal:  Infect Immun       Date:  1994-03       Impact factor: 3.441

6.  High-throughput synthesis and screening of cyclic peptide antibiotics.

Authors:  Qing Xiao; Dehua Pei
Journal:  J Med Chem       Date:  2007-06-05       Impact factor: 7.446

7.  Identification and characterization of a pore-forming protein of human peripheral blood natural killer cells.

Authors:  C C Liu; B Perussia; Z A Cohn; J D Young
Journal:  J Exp Med       Date:  1986-12-01       Impact factor: 14.307

8.  Properties of a purified pore-forming protein (perforin 1) isolated from H-2-restricted cytotoxic T cell granules.

Authors:  J D Young; E R Podack; Z A Cohn
Journal:  J Exp Med       Date:  1986-07-01       Impact factor: 14.307

9.  Zanvil Alexander Cohn 1926-1993.

Authors:  R M Steinman; C L Moberg
Journal:  J Exp Med       Date:  1994-01-01       Impact factor: 14.307

10.  Dissociation of membrane binding and lytic activities of the lymphocyte pore-forming protein (perforin).

Authors:  J D Young; A Damiano; M A DiNome; L G Leong; Z A Cohn
Journal:  J Exp Med       Date:  1987-05-01       Impact factor: 14.307
  10 in total

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