Taylor J Schoen1,2, Anna Huttenlocher1,3, Nancy P Keller1,4. 1. Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, Wisconsin. 2. Comparative Biomedical Sciences Graduate Program, University of Wisconsin-Madison, Madison, Wisconsin. 3. Department of Pediatrics, University of Wisconsin-Madison, Madison, Wisconsin. 4. Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin.
Abstract
The larval zebrafish is an increasingly popular host model for the study of Aspergillosis. The visual accessibility, genetic resources, small size, and ease of handling make zebrafish larvae compatible with higher-throughput investigation of fungal virulence and host resistance mechanisms. This article provides the protocols needed to prepare Aspergillus fumigatus spore inocula and use microinjection to infect the hindbrain ventricle of zebrafish larvae. Furthermore, we include protocols for analyzing host survival, immobilizing larvae for live imaging, and suggestions for image analysis.
The larval zebrafish is an increasingly popular host model for the study of Aspergillosis. The visual accessibility, genetic resources, small size, and ease of handling make zebrafish larvae compatible with higher-throughput investigation of fungal virulence and host resistance mechanisms. This article provides the protocols needed to prepare Aspergillus fumigatus spore inocula and use microinjection to infect the hindbrain ventricle of zebrafish larvae. Furthermore, we include protocols for analyzing host survival, immobilizing larvae for live imaging, and suggestions for image analysis.
Authors: Taylor J Schoen; Emily E Rosowski; Benjamin P Knox; David Bennin; Nancy P Keller; Anna Huttenlocher Journal: J Cell Sci Date: 2019-12-20 Impact factor: 5.285
Authors: Kayla Huemer; Jayne M Squirrell; Robert Swader; Danny C LeBert; Anna Huttenlocher; Kevin W Eliceiri Journal: Zebrafish Date: 2016-09-27 Impact factor: 1.985
Authors: Benjamin P Knox; Adriana Blachowicz; Jonathan M Palmer; Jillian Romsdahl; Anna Huttenlocher; Clay C C Wang; Nancy P Keller; Kasthuri Venkateswaran Journal: mSphere Date: 2016-10-26 Impact factor: 4.389
Authors: Emily E Rosowski; Nicholas Raffa; Benjamin P Knox; Netta Golenberg; Nancy P Keller; Anna Huttenlocher Journal: PLoS Pathog Date: 2018-08-02 Impact factor: 6.823